Molecular Features of Class II MHC-Restricted T-Cell Recognition of Protein and Peptide Antigens: The Importance of Amphipathic Structures

  • J. A. Berzofsky
  • J. Cornette
  • H. Margalit
  • I. Berkower
  • K. Cease
  • C. DeLisi
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 130)

Abstract

Much work has been done on the use of peptides as immunogens, but most has concentrated on antibody production (reviewed in LERNER 1984; ARNON 1984) rather than T-cell responses. There are certain potential problems with their use for antibody production that do not apply to T cells. Antibodies raised against peptides as immunogens generally cross-react with the native protein from which the peptides were derived with affinities several Orders of magnitude lower than those for the peptide (reviewed in BERZOFSKY 1985 a). Conversely, antibodies raised against the native protein generally cross-react with peptides derived from it with a lower affinity, although exceptions have been noted (LANDO and REICHLIN 1982). In both directions, cross-reactions seem to be most easily measured when the peptide corresponds to a more mobile portion of the protein (TAINER et al. 1984, 1985; WESTHOF et al. 1984); perhaps such portions are better able to share some conformational states with the peptide so that one can achieve an induced fit with antibodies made against the other (BERZOFSKY 1985 a). In addition, there are antibodies made against the native protein that react with assembled topographic determinants consisting of amino acid residues far apart in the primary sequence that are brought together on the surface of the native molecule as it folds in its native conformation (BENJAMIN et al. 1984; BERZOFSKY 1985a). Such antigenic sites exist only on the surface topography of the native protein, and thus do not exist in any peptide segment of the protein.

Keywords

HPLC Hepatitis Agar Chrome Bromide 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1986

Authors and Affiliations

  • J. A. Berzofsky
    • 1
  • J. Cornette
    • 1
  • H. Margalit
    • 1
  • I. Berkower
    • 1
  • K. Cease
    • 1
  • C. DeLisi
    • 1
  1. 1.Metabolism Branch and Laboratory of Mathematical BiologyNational Cancer Institute, National Institutes of HealthBethesdaUSA

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