Gastrin and Cholecystokinin

An Arduous Task for the Peptide Chemist
  • Luis Moroder
  • Erich Wünsch

Summary

Both during synthesis and storage as well as handling of gastrin- and cholecystokinin-peptides serious difficulties related to a sequence-dependent high reactivity of various side chain functions were encountered. Thus, methods were devised to bypass or at least control side reactions along the synthetic routes, and analogs were designed to enhance the stability of the peptide factors with concomitant retainment of full hormonal potency. The access of these gastrin- and cholecystokinin-peptides allowed intensive physiological, biological and conformational studies aimed at a better understanding of the mechanism of action of this class of hormones. Additional attention was paid to particular derivatives well suited to improve the immunochemical methods as needed for a deeper insight into the physiological significance of the remarkable heterogeneity of these gut hormones.

Keywords

Tryptophan Leucine Indole Glucagon Piperidine 

Abbreviations

Mox

methoxinine (oxa-analog of methionine)

DCC

dicylo-hexylcarbodiimide

HONSu

N-hydroxysuccinimide

HOBt

1-hy-droxybenzotriazole

DMF

dimethylformamide

NMP

N-methylpyr-rolidone

MEI

2-morpholinoethylisocyanide

CCK

cholecysto-kinin-pancreozymin

HG

human gastrin, whereby the additional number indicates the chain-length of the gastrin peptide

tlc

thin-layer chromatography

hplc

high performance liquid chromatography

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Copyright information

© Springer-Verlag Berlin Heidelberg 1986

Authors and Affiliations

  • Luis Moroder
    • 1
  • Erich Wünsch
    • 1
  1. 1.Abteilung PeptidchemieMax-Planck-Institut für BiochemieMartinsried bei MünchenGermany

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