The Mouse Egg as a Model in In Vitro Fertilization and Cryobiology

  • F. Leroy
  • A. Massip
  • F. Puissant
Conference paper

Abstract

Human in vitro fertilization (IVF), as many other medical advances, owes much to work performed on the laboratory mouse. It appears, for instance, that the vast majority of papers which have dealt with the composition and adjustment of media for mammalian IVF and embryo culture have been based on work with mice. Since the genetics of this species has become well-known, mouse eggs are also largely employed in experimental embryology and have become the tool of choice for mammalian transgenic experiments which are being carried out in an increasing number of laboratories [1]. In relation to clinical IVF, mouse embryos are extensively used for routine quality control of culture media and vessels.

Keywords

Permeability Sucrose Dioxide Glycerol Dehydration 

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References

  1. 1.
    Constantini F, Jaenisch R (eds) (1985) Genetic manipulation of the early mammalian embryo, 20 Banbury report. Cold Spring Harbor Laboratory, New YorkGoogle Scholar
  2. 2.
    Mohr L (1984) Assessment of human embryos. In: Trounson A, Wood C (eds) In vitro fertilization and embryo transfer. Livingstone, Edinburgh, p 159Google Scholar
  3. 3.
    Bowman P, Mc Laren A (1970) Cleavage rate of mouse embryos in vivo and in vitro. J Embryol Exp Morphol 24:203–207PubMedGoogle Scholar
  4. 4.
    Harlow GM, Quinn P (1979) Foetal and placental growth in the mouse after preimplantation development in vitro under oxygen concentrations of 5 and 20%. Aust J Biol Sci 32:363–369PubMedGoogle Scholar
  5. 5.
    Mc Reynolds HD, Hadek R (1972) A comparison of the fine structure of the late blastocyst developed in vivo and in vitro. J Exp Zool 182:95–118CrossRefGoogle Scholar
  6. 6.
    Renard JP, Heyman Y, Ozil JP (1980) Importance of gestation losses after non-surgical transfer of cultured and non-cultured bovine blastocysts. Vet Rec 107:152–153PubMedCrossRefGoogle Scholar
  7. 7.
    Massip A, Van der Zwalmen P, Puissant F, Camus M, Leroy F (1984) Effect of in vitro fertilization, culture, freezing and transfer on the ability of mouse embryos to implant and survive. J Reprod Fertil 71:199–204PubMedCrossRefGoogle Scholar
  8. 8.
    Degueldre M, Puissant F, Camus M, Tomberg A, Phan Thi H, Melot C, Leroy F (1984) Effect of carbon dioxide insufflation at laparoscopy on the gas phase in oocyte recovery fluids. J In Vitro Fertil Embryo Transfer 1:106–106Google Scholar
  9. 9.
    Gibori G, Rotschild I, Pepe GJ, Morishige WK, Lam P (1974) Luteotropic action of decidual tissue in the rat. Endocrinology 108:2060–2066CrossRefGoogle Scholar
  10. 10.
    Camus M, Puissant F, Deweze-Van Hoeck J, Leroy F (1985) Development and quality of embryos in relation to clinical characteristics of IVF trials. Abstracts handbook. 4th World conference on IVF, MelbourneGoogle Scholar
  11. 11.
    Puissant F, Lejeune B, Leroy F (1984) Effects on mature mouse oocytes of ultrasound treatment applied before in vitro fertilization. IRCS Med Sci 12:421–422Google Scholar
  12. 12.
    Bologne R, Demoulin A, Schaaps JP, Hustin J, Lambotte R (1983) CR Soc Biol (Paris) 177:381–387Google Scholar
  13. 13.
    Demoulin A, Schaaps JP, Bologne R, Lambotte R (1984) Is ultrasound monitoring of follicular growth harmless? J In Vitro Fertil Embryo Transfer 1:106–106Google Scholar
  14. 14.
    Schneider U, Hahn J, Salzer H (1974) Erste Ergebnisse der Tiefgefrierkonservierung von Mäuse- und Kanincheneizellen. Dtsch-Tieraerzth Wochenschr 81:470–472Google Scholar
  15. 15.
    Nakagata N, Toyoda Y (1980) Normal young after transfer of frozen-thawed 2-cell mouse embryos obtained by fertilization in vitro. Jpn J Zootechn Sci 51:740–744Google Scholar
  16. 16.
    Whittingham DG (1981) Viability assays. In: Zeilmaker GH (ed) Storage of laboratory animals. Fisher, Stuttgart, p 95Google Scholar
  17. 17.
    Cohen J, Simons RF, Edwards RG, Fehilly CB, Fishel SB (1985) Pregnancies following the frozen storage of storage of expanding human blastocysts. J In Vitro Fertil Embryo Transfer 2:59–64CrossRefGoogle Scholar
  18. 18.
    Mohr LR, Trounson A, Freeman L (1985) Deep freezing and transfer of human embryos. J In Fitro Fertil Embryo Transfer 2:1–10CrossRefGoogle Scholar
  19. 19.
    Kasai M, Niwa K, Iritani A (1980) Survival of mouse embryos frozen and thawed rapidly. J Reprod Fertil 59:51–56PubMedCrossRefGoogle Scholar
  20. 20.
    Miyamoto H, Ishibashi T (1983) Survival of mouse embryos frozen-thawed slowly or rapidly in the presence of various cryoprotectants. J Exp Zool 226:123–127PubMedCrossRefGoogle Scholar
  21. 21.
    Wood MJ, Farrant J (1980) Preservation of mouse embryos by two-step freezing. Cryobiology 17:178–180PubMedCrossRefGoogle Scholar
  22. 22.
    Massip A, Van Der Zwalmen P, Leroy F (1984) Effect of stage of development on survival of mouse embryos frozen thawed rapidly. Cryobiology 21:574–577PubMedCrossRefGoogle Scholar
  23. 23.
    Massip A, Van der Zwalmen P (1982) In vitro survival of mouse embryos frozen in glycerol or in glycerol-sucrose. Cryo-Letters 3:326–326Google Scholar
  24. 24.
    Leibo SP (1977) Fundamental cryobiology of mouse ova and embryos. In: Eliott K, Whelan J (eds) The freezing of mammalian embryos. Ciba Founation symposium Elsevier, Amsterdam, p 125Google Scholar
  25. 25.
    Testart LJ, Lasalle B, Belaisch-Allart, Hazout A, Rainhorn JP, Frydman R (to be published) High pregnancy rate after early human embryo freezing. Fertil SterilGoogle Scholar
  26. 26.
    Takeda T, Elsden RP, Seidel GE Jr (1984) Cryopreservation of mouse embryos by direct plunging into liquid nitrogen. Theriogenol 21:266–266 (abstract)CrossRefGoogle Scholar
  27. 27.
    Krag KT, Koehler IM, Wright RW Jr (1985) A method for freezing early murine embryos by plunging directly into liquid nitrogen. Theriogenol 23:199–199 (abstract)CrossRefGoogle Scholar
  28. 28.
    Williams TJ, Johnson SE (1985) Quick freezing of day 4 mouse embryos. Theriogenol 23:235–235 (abstract)CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1987

Authors and Affiliations

  • F. Leroy
    • 1
  • A. Massip
    • 2
  • F. Puissant
    • 1
  1. 1.Human Reproduction Research Unit and IVF Clinic, St Pierre HospitalFree University of BrusselsBelgium
  2. 2.Department of Obstetrics and Animal Reproduction, Faculty of Veterinary MedicineUniversity of LiègeBelgium

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