Fertilization by Microinjection of Human Sperm in Hamster and Human Oocytes
The interest of human sperm injection in the periovular space (SIPOS) is to study the fertilizing capacity of one or several human sperm selected on the basis of their phenotype (morphology and mobility). This approach can provide new information on the male gamete with the study of individual sperm rather than a heterogeneous sperm population.
KeywordsMigration Albumin Syringe Trypsin Infertility
Unable to display preview. Download preview PDF.
- Aitken RJ, Wang YF, Liu J, Best F, Richardson DW (1983) The influence of medium composition, osmolarity and albumin content on the acrosome reaction and fertilizing capacity of human spermatozoa. Development of an improved zona-free hamster egg penetration test. Int J Androl 6:180–193PubMedCrossRefGoogle Scholar
- Chan SYW, Fong WF, Chan PH, No PC (1985) Fertility promoting factor from human seminal plasma stimulates human spermatozoal fertilizing capacity in vitro. IRCS Med Sci 13:76–77Google Scholar
- Kanwar KC, Yanagimachi R, Lopata A (1973) Effects of human seminal plasma on fertilizing capacity of human spermatozoa. Fertil Steril 31 (3):321–327Google Scholar
- Lassalle B, Courtot AM, Testart J (1985) Fertilization of hamster oocyte by microinjection of human sperm in the perivitelline space. In: Testart J. Frydman R Ceds) Human in vitro fertilization — actual problems and prospects. Inserm-Elsevier, AmsterdamGoogle Scholar