Prognostic Significance of Viral Antigens in Liver Tissue

  • F. Callea
  • F. Facchetti
  • E. Bonera
  • G. Grasso Caprioli
  • M. Zorzi
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)

Abstract

Over the past 15 years, a number of observations have culminated in the localization of viral antigens in liver tissue by the use of routine histology, immunohistochemistry, electron microscopy (EM), and immuno-electron microscopy (IEM).

Keywords

Peroxi Hepatitis Paraffin Bismuth Hematoxylin 

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References

  1. Alberti A, Trevisan A, Fattovich G, Realdi G (1984) The role of hepatitis B virus replication and hepatocyte membrane expression in the pathogenesis of HBV-related hepatic damage. In: Chisari FV (ed) Advances in hepatitis research. Masson, New York, pp 134–143Google Scholar
  2. Arnold W, Nielsen JO, Hardts F, Meyer zum Buschenfelde KH (1977) Localization of e-antigen in nuclei of hepatocytes in HBsAg-positive liver disease. Gut 19: 994–996CrossRefGoogle Scholar
  3. Bianchi L, Gudat F (1976) Sanded nuclei in hepatitis B. Eosinophilic inclusions in liver cell nuclei due to excess in hepatitis B core antigen formation. Lab Invest 35: 1–5PubMedGoogle Scholar
  4. Bianchi L, Gudat F (1979) Immunopathology of hepatitis B. In: Popper H, Schaffner F (eds) Progress in liver disease, vol VI. Grune & Stratton, New York, pp 371–392Google Scholar
  5. Bonino F, Negro F, Tardanico R, Callea F, Verme G, Rizzetto M (1985) Chronic anti-HBe hepatitis with serum HBV-DNA. A separate entitiy. Hepatology 4: 1041Google Scholar
  6. Bonino F, Rosina F, Rizzetto M, Rizzi R, Chiaberge E, Tardanico R, Callea F, Verme G (1986) Chronic hepatitis in HBsAg carriers with serum HBV-DNA and anti-HBe. Gastroenterology (in press)Google Scholar
  7. Brechot, Degos F, Lugassy C, Thiers V, Zafrani S, Franco D, Bismuth H, Trepo C, Benhamou JP, Wands J, Isselbacher K, Tiollais P, Berthelot P (1985) Hepatitis B virus DNA in patients with chronic liver disease and negative tests for hepatitis B surface antigen. N Engl J Med 312: 270–276PubMedCrossRefGoogle Scholar
  8. Callea F (1983) Immunohistochemical study on alpha-l-antitrypsin. Thesis Acco, Leuven, pp 7886Google Scholar
  9. Callea F, De Vos R, Togni R, Tardanico R, Vanstapel MJ, Desmet VJ (1986) Fibrinogen inclusions in liver cells: a new type of ground-glass hepatocyte. Immune light and electron microscopic characterization. Histopathology 10: 65–73PubMedCrossRefGoogle Scholar
  10. Callea F, Fevery J, De Groote J, Desmet VJ (1986) Detection of Pi Z phenotype individuals by alpha-l-antitrypsin ( AAT) immunohistochemistry in paraffin embedded liver specimens. J Hepatology 2: 389–401CrossRefGoogle Scholar
  11. Coyne (Zavatone) VE, Millman I, Cerda J, Gerstley BJS, London T, Sutnick A, Blumberb BS (1970) The localization of Australia Antigen by immunofluorescence. J Exp Med 131: 307–325PubMedCrossRefGoogle Scholar
  12. Canese MG, Rizzetto M, Aricò S, Crivelli O, Zanetti AR, Macchiorlati E, Ponzetto A, Leone L, Mollo F, Verme G (1979) An ultrastructural and immunohistochemical study on the antigen associated with the hepatitis B virus. J Pathol 128: 169–175PubMedCrossRefGoogle Scholar
  13. De Vos R, Ray MB, Desmet VJ (1978) Electron microscopy of hepatitis B virus components in chronic active liver disease. J Clin Pathol 32: 590–600CrossRefGoogle Scholar
  14. De Vos R, Vanstapel MJ, Desmyter J, De Wolf-Peeters, De Groote G, Colaert J. Mortelmnans J, De Groote J, Fevery J, Desmet V (1983) Are nuclear particles specific for non-A, non-B hepatitis? Hepatology 3: 532–544PubMedCrossRefGoogle Scholar
  15. Dunn AEG, Peters RL, Schwaitzer IL, Spears RL (1972) Virus-like particles in livers of infants with vertically transmitted hepatitis. Arch Pathol 94: 258PubMedGoogle Scholar
  16. Edgington GS, Chisari FV (1975) Immunological aspects of hepatitis B virus infection. Am J Med Sci 270: 213–227CrossRefGoogle Scholar
  17. Facchetti F, Bonera E, Albertini A, Zorzi M, Callea F (1985) Immunohistochemical patterns of HBcAg, HLA class I and II in different liver disease with hepatocytic membrane expression of HBsAg. Hepatology 5: 1049Google Scholar
  18. Facchetti F, Tardanico R, Bonetti MF, Guerini A, Callea F (1986) HBsAg, HBcAg and Delta-Ag in liver tissue. Simultaneous visualization in a single tissue section by triple immunostaining. Histology and Histopathology 1: 181–185PubMedGoogle Scholar
  19. Furuta S, Kiyosawa K, Nagata A, Akahane Y, Oda M (1975) HBsAg on cell membrane in sympthom free carrier. Lancet 11: 227CrossRefGoogle Scholar
  20. Gowans EJ, Burrel CJ (1985) Widespread presence of cytoplasmic HBcAg in hepatitis B infected liver detected by improved immunochemical methods. J Clin Pathol 38: 393–398PubMedCrossRefGoogle Scholar
  21. Gudat F, Bianchi L (1977a) Evidence for phasic sequence in nuclear HBcAg formation and cell membrane-directed flow of core particles in chronic hepatitis B. Gastroenterology 73: 1194–1197PubMedGoogle Scholar
  22. Gudat F, Bianchi L (1977b) HBsAg: A target antigen on the liver cell? In: Popper H, Bianchi L (eds) Membrane alterations as basis of liver injury. MTP Press, Lancaster, pp 171–178Google Scholar
  23. Hadziyannis S, Gerber MA, Vissoulis C, Popper H (1973) Cytoplasmic hepatitis B antigen in “ground-glass” hepatocytes of carriers. Arch Pathol 96: 327–330PubMedGoogle Scholar
  24. Hodges JR, Millward-Sadler GH, Barbatis C, Wright R (1981) Heterozygous MZ alpha-l-antitrypsin deficiency in adults with chronic active hepatitis and cryptogenic cirrhosis. N Engi J Med 304: 447–560CrossRefGoogle Scholar
  25. Hsu SM, Raine L, Fanger H (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques. A comparison between ABC and unlabeled antibody ( PAP) procedures. J Histochem Cytochem 29: 577–580PubMedCrossRefGoogle Scholar
  26. Huang SN, Neurath AR (1979) Immunohistologic demonstration of hepatitis B viral antigens in liver with reference to its significance in liver injury. Lab Invest 40: 1–17PubMedGoogle Scholar
  27. Kamimura T, Ponzetto A, Bonino F, Feinstone SM, Gerin JL, Purcell RH (1983) Cytoplasmic tubular structures in liver of HBsAg Carrier chimpanzees infected with Delta agent and comparison with cytoplasmic structures in non-A, non-B hepatitis. Hepatology 3: 631–637PubMedCrossRefGoogle Scholar
  28. Kojima T (1982) Immune electron microscopic study of hepatitis B virus associated antigens in hepatocytes. Gastroenterol Jpn 17: 558–575Google Scholar
  29. Kojima T, Desmet VJ (1984) Hepatitis B core antigen (HBcAg) in liver cell plasma membrane: immunoelectron microscopic study. Meeting IASL Berne Sept 3–4 (Abstr) 122Google Scholar
  30. Kojima T, Callea F, Desmyter J, Desmet VJ (1985) Immunoelectron microscopic study of hepatitis Delta antigen ( HDAg) in hepatocytes. Hepatology 5: 957Google Scholar
  31. Lock ASF, Lindsay I, Scheuer PJ, Thomas MC (1985) Clinical and histological features of delta infection in chronic hepatitis B virus carriers. J Clin Pathol 38: 530–533CrossRefGoogle Scholar
  32. Meyer zum Buschenfelde KH, Hütteroth TH, Arnold W, Hopf U (1979) Immunologic liver injury: The role of hepatitis B viral antigens and liver membrane antigens as targets. In: Popper H, Schaffner F (eds) Progress in liver disease, vol VI. Grune & Stratton, New York, pp 407–424Google Scholar
  33. Mondelli M, Naumov N, Eddlestone ALF (1984) The immunopathogenesis of liver cell damage in chronic hepatitis B virus infection. In: Chisari FV (ed) Advances in hepatitis research. Masson, New York, pp 144–151Google Scholar
  34. Negro F, Chiaberge E, Oliviero S, Hammer M, Berninger M, Canese MG, Bonino F (1984) Hepatitis B virus DNA ( HBV-DNA) in anti-HBe positive sera. Liver 4: 177–183PubMedGoogle Scholar
  35. Nowoslawski A, Brzosko WJ, Madalinski K, Krawczynski K (1970) Cellular localization of Australia antigen in the liver of patients with lymphoproliferative disorders. Lancet 1: 494–498PubMedCrossRefGoogle Scholar
  36. Nowoslawski A, Krawczynski K, Nazarewicz T, Slusarczyk J (1975) Immunopathological aspects of hepatitis type B. Am J Med Sci 270: 229–239PubMedCrossRefGoogle Scholar
  37. Popper H, Thung SN, Gerber MA, Hadler SC, De Monzon M, Ponzetto A, Anzola E, Rivera D, Mondolfi A, Bracho A, Francis DP, Gerin JL, Maynard JE, Purcell RH (1983) Histologic studies of severe Delta antigen infection in Venezuelan Indians. Hepatology 3: 906–912PubMedCrossRefGoogle Scholar
  38. Ray MB, Desmet VJ, Bradburne AF, Fevery J, Desmyter J, De Groote J (1976) Differential distribution of hepatitis B surface antigen and hepatitis B core antigen in the liver of hepatitis B patients. Gastroenterology 71: 462–467PubMedGoogle Scholar
  39. Rizzetto M, Canese MG, Aricó S, Crivelli O, Trepo C, Bonino F, Verme G (1977) Immunofluorescent detection of new antigen-antibody system (ö/anti-6) associated to Hepatitis B virus in liver and serum of HBsAg carriers. Gut 18: 997–1003PubMedCrossRefGoogle Scholar
  40. Rizzetto M, Verme G, Recchia S, Bonino F, Farci P, Arid S, Calzia R, Picciotto A, Colombo M, Popper H (1983) Chronic HBsAg hepatitis with intrahepatic expression of Delta antigen. An active and progressive disease unresponsive to immunosuppressive treatment. Ann Intern Med 98: 437–441PubMedGoogle Scholar
  41. Rizzetto M (1983) The Delta agent. Hepatology 3: 729–737PubMedCrossRefGoogle Scholar
  42. Schweitzer IL, Dunn AEG, Peters RL, Spears RL (1973) Viral hepatitis B in neonats and infants. Am J Med 55: 762–771PubMedCrossRefGoogle Scholar
  43. Shikata T, Uzawa T, Yoshiwara N, Akatsuka T, Yamazaki S (1974) Staining methods of Australia antigen in paraffin section. Detection of cytoplasmic inclusion bodies. Jpn Exp Med 44: 25–36Google Scholar
  44. Smedile A, Farci P, Verme G, Caredda F, Cargnel A, Caporaso N, Dentico P, Trepo C, Opolon P, Gimson A, Vergani D, Williams R, Rizzetto M (1982) Influence of delta infection on severity of hepatitis B. Lancet 2: 945–947PubMedCrossRefGoogle Scholar
  45. Tanaka K, Mori W, Suwa S (1981) Victoria blue-nuclear fast red stain for HBsAg antigen detection in paraffin section. Acta Pathol Jpn 31: 93–98PubMedGoogle Scholar
  46. Tardanico R, Zorzi F, Bonetti F, Guerini A, Albertini A, Zorzi M, Rizzetto M, Callea F (1985) Distribution patterns of HBsAg and HBcAg in Delta-Ag positive liver biopsies. Hepatology 5: 1048Google Scholar
  47. Trepo C, Vitviski L, Neurath R, Hashimoto N, Schaefer R, Nemoz G, Prince AM (1976) Detection of e antigen by immunofluorescence in cytoplasm of hepatocytes of HBsAg carriers. Lancet 1: 486PubMedCrossRefGoogle Scholar
  48. Yamada G, Nakane PK (1977) Hepatitis B core and surface antigens in liver tissue: light and electron microscopic localization by the peroxidase-labeled antibody method. Lab Invest 36: 649–659PubMedGoogle Scholar
  49. Yamada G, Feinberg LE, Nakane PK (1978) Hepatitis B cytologic localization of virus antigens and the role of the immune response. Hum Pathol 9: 93PubMedCrossRefGoogle Scholar
  50. Verme G, Rocca G, Rizzi R, Mollo F, David E, Solcia E, Sessa F (1983) Histopathology of chronic delta hepatitis. In: Verme G, Bonino F, Rizzetto M (eds) Viral hepatitis and delta infection. Liss, New York, pp 169–176Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1986

Authors and Affiliations

  • F. Callea
  • F. Facchetti
  • E. Bonera
  • G. Grasso Caprioli
  • M. Zorzi
    • 1
  1. 1.Histochemistry Unit 1st Department of Pathology, and 3rd Department of Internal MedicineSpedali Civili of BresciaBresciaItaly

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