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Prognostic Significance of Viral Antigens in Liver Tissue

  • Conference paper
Viral Hepatitis

Part of the book series: Proceedings in Life Sciences ((LIFE SCIENCES))

Abstract

Over the past 15 years, a number of observations have culminated in the localization of viral antigens in liver tissue by the use of routine histology, immunohistochemistry, electron microscopy (EM), and immuno-electron microscopy (IEM).

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References

  • Alberti A, Trevisan A, Fattovich G, Realdi G (1984) The role of hepatitis B virus replication and hepatocyte membrane expression in the pathogenesis of HBV-related hepatic damage. In: Chisari FV (ed) Advances in hepatitis research. Masson, New York, pp 134–143

    Google Scholar 

  • Arnold W, Nielsen JO, Hardts F, Meyer zum Buschenfelde KH (1977) Localization of e-antigen in nuclei of hepatocytes in HBsAg-positive liver disease. Gut 19: 994–996

    Article  Google Scholar 

  • Bianchi L, Gudat F (1976) Sanded nuclei in hepatitis B. Eosinophilic inclusions in liver cell nuclei due to excess in hepatitis B core antigen formation. Lab Invest 35: 1–5

    PubMed  CAS  Google Scholar 

  • Bianchi L, Gudat F (1979) Immunopathology of hepatitis B. In: Popper H, Schaffner F (eds) Progress in liver disease, vol VI. Grune & Stratton, New York, pp 371–392

    Google Scholar 

  • Bonino F, Negro F, Tardanico R, Callea F, Verme G, Rizzetto M (1985) Chronic anti-HBe hepatitis with serum HBV-DNA. A separate entitiy. Hepatology 4: 1041

    Google Scholar 

  • Bonino F, Rosina F, Rizzetto M, Rizzi R, Chiaberge E, Tardanico R, Callea F, Verme G (1986) Chronic hepatitis in HBsAg carriers with serum HBV-DNA and anti-HBe. Gastroenterology (in press)

    Google Scholar 

  • Brechot, Degos F, Lugassy C, Thiers V, Zafrani S, Franco D, Bismuth H, Trepo C, Benhamou JP, Wands J, Isselbacher K, Tiollais P, Berthelot P (1985) Hepatitis B virus DNA in patients with chronic liver disease and negative tests for hepatitis B surface antigen. N Engl J Med 312: 270–276

    Article  PubMed  CAS  Google Scholar 

  • Callea F (1983) Immunohistochemical study on alpha-l-antitrypsin. Thesis Acco, Leuven, pp 7886

    Google Scholar 

  • Callea F, De Vos R, Togni R, Tardanico R, Vanstapel MJ, Desmet VJ (1986) Fibrinogen inclusions in liver cells: a new type of ground-glass hepatocyte. Immune light and electron microscopic characterization. Histopathology 10: 65–73

    Article  PubMed  CAS  Google Scholar 

  • Callea F, Fevery J, De Groote J, Desmet VJ (1986) Detection of Pi Z phenotype individuals by alpha-l-antitrypsin ( AAT) immunohistochemistry in paraffin embedded liver specimens. J Hepatology 2: 389–401

    Article  CAS  Google Scholar 

  • Coyne (Zavatone) VE, Millman I, Cerda J, Gerstley BJS, London T, Sutnick A, Blumberb BS (1970) The localization of Australia Antigen by immunofluorescence. J Exp Med 131: 307–325

    Article  PubMed  CAS  Google Scholar 

  • Canese MG, Rizzetto M, Aricò S, Crivelli O, Zanetti AR, Macchiorlati E, Ponzetto A, Leone L, Mollo F, Verme G (1979) An ultrastructural and immunohistochemical study on the antigen associated with the hepatitis B virus. J Pathol 128: 169–175

    Article  PubMed  CAS  Google Scholar 

  • De Vos R, Ray MB, Desmet VJ (1978) Electron microscopy of hepatitis B virus components in chronic active liver disease. J Clin Pathol 32: 590–600

    Article  Google Scholar 

  • De Vos R, Vanstapel MJ, Desmyter J, De Wolf-Peeters, De Groote G, Colaert J. Mortelmnans J, De Groote J, Fevery J, Desmet V (1983) Are nuclear particles specific for non-A, non-B hepatitis? Hepatology 3: 532–544

    Article  PubMed  Google Scholar 

  • Dunn AEG, Peters RL, Schwaitzer IL, Spears RL (1972) Virus-like particles in livers of infants with vertically transmitted hepatitis. Arch Pathol 94: 258

    PubMed  CAS  Google Scholar 

  • Edgington GS, Chisari FV (1975) Immunological aspects of hepatitis B virus infection. Am J Med Sci 270: 213–227

    Article  Google Scholar 

  • Facchetti F, Bonera E, Albertini A, Zorzi M, Callea F (1985) Immunohistochemical patterns of HBcAg, HLA class I and II in different liver disease with hepatocytic membrane expression of HBsAg. Hepatology 5: 1049

    Google Scholar 

  • Facchetti F, Tardanico R, Bonetti MF, Guerini A, Callea F (1986) HBsAg, HBcAg and Delta-Ag in liver tissue. Simultaneous visualization in a single tissue section by triple immunostaining. Histology and Histopathology 1: 181–185

    PubMed  CAS  Google Scholar 

  • Furuta S, Kiyosawa K, Nagata A, Akahane Y, Oda M (1975) HBsAg on cell membrane in sympthom free carrier. Lancet 11: 227

    Article  Google Scholar 

  • Gowans EJ, Burrel CJ (1985) Widespread presence of cytoplasmic HBcAg in hepatitis B infected liver detected by improved immunochemical methods. J Clin Pathol 38: 393–398

    Article  PubMed  CAS  Google Scholar 

  • Gudat F, Bianchi L (1977a) Evidence for phasic sequence in nuclear HBcAg formation and cell membrane-directed flow of core particles in chronic hepatitis B. Gastroenterology 73: 1194–1197

    PubMed  CAS  Google Scholar 

  • Gudat F, Bianchi L (1977b) HBsAg: A target antigen on the liver cell? In: Popper H, Bianchi L (eds) Membrane alterations as basis of liver injury. MTP Press, Lancaster, pp 171–178

    Google Scholar 

  • Hadziyannis S, Gerber MA, Vissoulis C, Popper H (1973) Cytoplasmic hepatitis B antigen in “ground-glass” hepatocytes of carriers. Arch Pathol 96: 327–330

    PubMed  CAS  Google Scholar 

  • Hodges JR, Millward-Sadler GH, Barbatis C, Wright R (1981) Heterozygous MZ alpha-l-antitrypsin deficiency in adults with chronic active hepatitis and cryptogenic cirrhosis. N Engi J Med 304: 447–560

    Article  Google Scholar 

  • Hsu SM, Raine L, Fanger H (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques. A comparison between ABC and unlabeled antibody ( PAP) procedures. J Histochem Cytochem 29: 577–580

    Article  PubMed  CAS  Google Scholar 

  • Huang SN, Neurath AR (1979) Immunohistologic demonstration of hepatitis B viral antigens in liver with reference to its significance in liver injury. Lab Invest 40: 1–17

    PubMed  CAS  Google Scholar 

  • Kamimura T, Ponzetto A, Bonino F, Feinstone SM, Gerin JL, Purcell RH (1983) Cytoplasmic tubular structures in liver of HBsAg Carrier chimpanzees infected with Delta agent and comparison with cytoplasmic structures in non-A, non-B hepatitis. Hepatology 3: 631–637

    Article  PubMed  CAS  Google Scholar 

  • Kojima T (1982) Immune electron microscopic study of hepatitis B virus associated antigens in hepatocytes. Gastroenterol Jpn 17: 558–575

    Google Scholar 

  • Kojima T, Desmet VJ (1984) Hepatitis B core antigen (HBcAg) in liver cell plasma membrane: immunoelectron microscopic study. Meeting IASL Berne Sept 3–4 (Abstr) 122

    Google Scholar 

  • Kojima T, Callea F, Desmyter J, Desmet VJ (1985) Immunoelectron microscopic study of hepatitis Delta antigen ( HDAg) in hepatocytes. Hepatology 5: 957

    Google Scholar 

  • Lock ASF, Lindsay I, Scheuer PJ, Thomas MC (1985) Clinical and histological features of delta infection in chronic hepatitis B virus carriers. J Clin Pathol 38: 530–533

    Article  Google Scholar 

  • Meyer zum Buschenfelde KH, Hütteroth TH, Arnold W, Hopf U (1979) Immunologic liver injury: The role of hepatitis B viral antigens and liver membrane antigens as targets. In: Popper H, Schaffner F (eds) Progress in liver disease, vol VI. Grune & Stratton, New York, pp 407–424

    Google Scholar 

  • Mondelli M, Naumov N, Eddlestone ALF (1984) The immunopathogenesis of liver cell damage in chronic hepatitis B virus infection. In: Chisari FV (ed) Advances in hepatitis research. Masson, New York, pp 144–151

    Google Scholar 

  • Negro F, Chiaberge E, Oliviero S, Hammer M, Berninger M, Canese MG, Bonino F (1984) Hepatitis B virus DNA ( HBV-DNA) in anti-HBe positive sera. Liver 4: 177–183

    PubMed  CAS  Google Scholar 

  • Nowoslawski A, Brzosko WJ, Madalinski K, Krawczynski K (1970) Cellular localization of Australia antigen in the liver of patients with lymphoproliferative disorders. Lancet 1: 494–498

    Article  PubMed  CAS  Google Scholar 

  • Nowoslawski A, Krawczynski K, Nazarewicz T, Slusarczyk J (1975) Immunopathological aspects of hepatitis type B. Am J Med Sci 270: 229–239

    Article  PubMed  CAS  Google Scholar 

  • Popper H, Thung SN, Gerber MA, Hadler SC, De Monzon M, Ponzetto A, Anzola E, Rivera D, Mondolfi A, Bracho A, Francis DP, Gerin JL, Maynard JE, Purcell RH (1983) Histologic studies of severe Delta antigen infection in Venezuelan Indians. Hepatology 3: 906–912

    Article  PubMed  CAS  Google Scholar 

  • Ray MB, Desmet VJ, Bradburne AF, Fevery J, Desmyter J, De Groote J (1976) Differential distribution of hepatitis B surface antigen and hepatitis B core antigen in the liver of hepatitis B patients. Gastroenterology 71: 462–467

    PubMed  CAS  Google Scholar 

  • Rizzetto M, Canese MG, Aricó S, Crivelli O, Trepo C, Bonino F, Verme G (1977) Immunofluorescent detection of new antigen-antibody system (ö/anti-6) associated to Hepatitis B virus in liver and serum of HBsAg carriers. Gut 18: 997–1003

    Article  PubMed  CAS  Google Scholar 

  • Rizzetto M, Verme G, Recchia S, Bonino F, Farci P, Arid S, Calzia R, Picciotto A, Colombo M, Popper H (1983) Chronic HBsAg hepatitis with intrahepatic expression of Delta antigen. An active and progressive disease unresponsive to immunosuppressive treatment. Ann Intern Med 98: 437–441

    PubMed  CAS  Google Scholar 

  • Rizzetto M (1983) The Delta agent. Hepatology 3: 729–737

    Article  PubMed  CAS  Google Scholar 

  • Schweitzer IL, Dunn AEG, Peters RL, Spears RL (1973) Viral hepatitis B in neonats and infants. Am J Med 55: 762–771

    Article  PubMed  CAS  Google Scholar 

  • Shikata T, Uzawa T, Yoshiwara N, Akatsuka T, Yamazaki S (1974) Staining methods of Australia antigen in paraffin section. Detection of cytoplasmic inclusion bodies. Jpn Exp Med 44: 25–36

    CAS  Google Scholar 

  • Smedile A, Farci P, Verme G, Caredda F, Cargnel A, Caporaso N, Dentico P, Trepo C, Opolon P, Gimson A, Vergani D, Williams R, Rizzetto M (1982) Influence of delta infection on severity of hepatitis B. Lancet 2: 945–947

    Article  PubMed  CAS  Google Scholar 

  • Tanaka K, Mori W, Suwa S (1981) Victoria blue-nuclear fast red stain for HBsAg antigen detection in paraffin section. Acta Pathol Jpn 31: 93–98

    PubMed  CAS  Google Scholar 

  • Tardanico R, Zorzi F, Bonetti F, Guerini A, Albertini A, Zorzi M, Rizzetto M, Callea F (1985) Distribution patterns of HBsAg and HBcAg in Delta-Ag positive liver biopsies. Hepatology 5: 1048

    Google Scholar 

  • Trepo C, Vitviski L, Neurath R, Hashimoto N, Schaefer R, Nemoz G, Prince AM (1976) Detection of e antigen by immunofluorescence in cytoplasm of hepatocytes of HBsAg carriers. Lancet 1: 486

    Article  PubMed  CAS  Google Scholar 

  • Yamada G, Nakane PK (1977) Hepatitis B core and surface antigens in liver tissue: light and electron microscopic localization by the peroxidase-labeled antibody method. Lab Invest 36: 649–659

    PubMed  CAS  Google Scholar 

  • Yamada G, Feinberg LE, Nakane PK (1978) Hepatitis B cytologic localization of virus antigens and the role of the immune response. Hum Pathol 9: 93

    Article  PubMed  CAS  Google Scholar 

  • Verme G, Rocca G, Rizzi R, Mollo F, David E, Solcia E, Sessa F (1983) Histopathology of chronic delta hepatitis. In: Verme G, Bonino F, Rizzetto M (eds) Viral hepatitis and delta infection. Liss, New York, pp 169–176

    Google Scholar 

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Author information

Authors and Affiliations

  1. Histochemistry Unit 1st Department of Pathology, and 3rd Department of Internal Medicine, Spedali Civili of Brescia, 25100, Brescia, Italy

    M. Zorzi

Authors
  1. F. Callea
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  2. F. Facchetti
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  3. E. Bonera
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  4. G. Grasso Caprioli
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  5. M. Zorzi
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Editor information

Editors and Affiliations

  1. Histochemistry Unit, 1st Department of Pathology Spedali Civili of Brescia, Aggregaat Hoger Onderwijs, Catholic University of Leuven, 25100, Brescia, Italy

    Francesco Callea

  2. 1st Department of Pathology, Spedali Civili of Brescia, 25100, Brescia, Italy

    Mario Zorzi (Professor of Pathology and Chairman) (Professor of Pathology and Chairman)

  3. Laboratory of Histochemistry and Cytochemistry, Catholic University of Leuven, 3000, Leuven, Belgium

    Valeer J. Desmet (Professor of Pathology) (Professor of Pathology)

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© 1986 Springer-Verlag Berlin Heidelberg

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Callea, F., Facchetti, F., Bonera, E., Caprioli, G.G., Zorzi, M. (1986). Prognostic Significance of Viral Antigens in Liver Tissue. In: Callea, F., Zorzi, M., Desmet, V.J. (eds) Viral Hepatitis. Proceedings in Life Sciences. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-71350-7_6

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  • DOI: https://doi.org/10.1007/978-3-642-71350-7_6

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-71352-1

  • Online ISBN: 978-3-642-71350-7

  • eBook Packages: Springer Book Archive

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