Our investigation revealed that by means of carbohydrate ultracytochemistry, a coat of glycoconjugates can be demonstrated on the inner surface of lysosomal and secretory granule membranes in a large variety of tissues from rat and mouse. This coat covers the interior, i.e., anticytoplasmic, surface of all intracellular membranes that are (a) derived from the Golgi apparatus, and (b) linked to the plasmalemma and to each other via the membrane flow associated with exocytosis, endocytosis, and crinophagy. On the other hand, mitochondrial and peroxisomal membranes not incorporated in this membrane flow lack a membrane coat.
KeywordsCarbohydrate Aldehyde Fractionation Dehydration Luminal
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