Evidence for a Link Between Peroxisomes and the Nonspecific Lipid Transfer Protein (Sterol Carrier Protein 2) in Rat and Human Liver

  • A. Van Amerongen
  • J. B. Helms
  • M. Van Noort
  • F. F. G. Rommerts
  • K. W. A. Wirtz
Conference paper
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)

Abstract

Antibodies against the nonspecific lipid transfer protein (nsL-TP) are cross-reactive with a high-molecular weight protein(s) found in the 105,000 x g supernatant and peroxisomes from rat and human liver. Levels of nsL-TP in Morris hepatoma 7777 and in the liver of Zellweger patients are greatly reduced as compared to controls (i.e. ten- to twenty-fold reduction). These tissues which are deficient in peroxisomes, do contain the high-molecular weight protein (s). This suggests that the occurrence of nsL-TP may depend on the presence of peroxisomes.

Levels of nsL-TP in the Leydig cells of rat testis are under hormonal control. Stimulation of rat tumor Leydig cells with luteinizing hormone doubled the levels of nsL-TP within two minutes under conditions where protein synthesis was inhibited. It remains to be established whether this post-translational processing involves cleavage of a high-molecular weight protein.

Keywords

Cholesterol Fractionation Cycloheximide Spiro Pregnenolone 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1987

Authors and Affiliations

  • A. Van Amerongen
    • 1
  • J. B. Helms
    • 1
  • M. Van Noort
    • 2
  • F. F. G. Rommerts
    • 2
  • K. W. A. Wirtz
    • 1
  1. 1.Laboratory of BiochemistryState UniversityUtrechtThe Netherlands
  2. 2.Department of Biochemistry (Division of Chemical Endocrinology)Erasmus UniversityRotterdamThe Netherlands

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