Induction of Cytochrome P-450 and Peroxisome Proliferation in Rat Liver by Perfluorinated Octane Sulphonic Acid (PFOS)
Perfluorinated octane sulphonic acid (PFOS), the unmetabolizable compound, induced the peroxisome proliferation significantly in rat liver. The common chemical structure of peroxisome proliferators was discussed. After administration of powdered chow containing 0.02%-PFOS to male rats of Wistar-Imamichi strain for 2 weeks, hepatic activities of catalase, cyanogen-insensitive fatty acyl-CoA oxidizing system (FAOS) and carnitine acetyl transferase (CAT) increased 1.74-, 4.90- and 6.84-fold, respectively. The significant induction of 80K-protein, the peroxisomal enoyl-CoA hydratase, was also observed by SDS-polyacrylamide gel electrophoresis. The prominent induction of peroxisome proliferation was demonstrated in electron micrograph. PFOS induced cytochrome P-450 which catalyzes co-hydroxylation of lauric acid, simultaneously. The effects of PFOS on other drug-metabolizing enzyme activities were moderate. Thus, the enhancement of fatty acid metabolism through dicarboxylic acid pathway was indicated.
KeywordsLauric Acid Erucic Acid Peroxisome Proliferation Carnitine Acetyl Transferase Powdered Chow
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