Abstract
Bacterial genomes cannot be purified as a stable, compact DNA-protein complex like chromatin. They can be isolated intact in a compact, supercoiled form from Bacillus species (Sloof et al. 1983), but the forces which maintain a compact conformation are easily disrupted, preventing extensive purification. Chromosomes from E. coli have only been isolated in an artificially compacted form. Compaction has been induced, for example, by addition of polyamines (Kornberg et al. 1974) or of large amounts of basic proteins like lysozyme (Guillen et al. 1978), or by using high salt concentrations (Stonington and Pettijohn 1971). These preparations of intact chromosomes have been too crude to identify the proteins which keep the bacterial chromosomes in vivo in a compact form. The well-documented negative superhelicity of the bacterial DNA, part of which appears not to be stabilised (Sinden et al. 1980), will be responsible for a large part of the compaction of the chromosome. There must be other factors, however, presumably mainly nucleic acid-protein interactions (Sloof et al. 1983), resulting in a further level of compaction.
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© 1986 Springer-Verlag Berlin Heidelberg
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Schouten, J.P. (1986). Detection and Identification of Proteins Cross-Linked in Vivo to Nucleic Acids. In: Gualerzi, C.O., Pon, C.L. (eds) Bacterial Chromatin. Proceedings in Life Sciences. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-71266-1_8
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DOI: https://doi.org/10.1007/978-3-642-71266-1_8
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