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Characterization of Bacillus subtilis Nucleoid DNA-Binding Proteins

  • F. Le Hégarat
  • V. Salti
  • L. Hirschbein
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)

Abstract

The tertiary structure of the bacterial genome has now been studied to some extent, mostly in the Gram-negative bacterium Escherichia coli, and various small basic proteins called “histonelike proteins” have been described by several authors. In the Gram-positive sporulating bacterium Bacillus subtilis, proteins together with RNA are also essential for the structural integrity of the isolated nucleoid. This work was focused on the identification and characterization of a group of nucleoid-associated proteins displaying some properties shown by the histones, i.e. heat stability, acid solubility and abundance in the cell.

Keywords

Bacillus Subtilis S100 Extract Nucleoid Protein Bacterial Nucleoid Phosphocellulose Chromatography 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1986

Authors and Affiliations

  • F. Le Hégarat
  • V. Salti
  • L. Hirschbein
    • 1
  1. 1.Institut de Microbiologie, Bat. 409Université Paris-SudOrsay CedexFrance

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