Characterization of Bacillus subtilis Nucleoid DNA-Binding Proteins

  • F. Le Hégarat
  • V. Salti
  • L. Hirschbein
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)


The tertiary structure of the bacterial genome has now been studied to some extent, mostly in the Gram-negative bacterium Escherichia coli, and various small basic proteins called “histonelike proteins” have been described by several authors. In the Gram-positive sporulating bacterium Bacillus subtilis, proteins together with RNA are also essential for the structural integrity of the isolated nucleoid. This work was focused on the identification and characterization of a group of nucleoid-associated proteins displaying some properties shown by the histones, i.e. heat stability, acid solubility and abundance in the cell.


Bacillus Subtilis S100 Extract Nucleoid Protein Bacterial Nucleoid Phosphocellulose Chromatography 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1986

Authors and Affiliations

  • F. Le Hégarat
  • V. Salti
  • L. Hirschbein
    • 1
  1. 1.Institut de Microbiologie, Bat. 409Université Paris-SudOrsay CedexFrance

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