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Characterization of Bacillus subtilis Nucleoid DNA-Binding Proteins

  • Conference paper
Bacterial Chromatin

Part of the book series: Proceedings in Life Sciences ((LIFE SCIENCES))

Abstract

The tertiary structure of the bacterial genome has now been studied to some extent, mostly in the Gram-negative bacterium Escherichia coli, and various small basic proteins called “histonelike proteins” have been described by several authors. In the Gram-positive sporulating bacterium Bacillus subtilis, proteins together with RNA are also essential for the structural integrity of the isolated nucleoid. This work was focused on the identification and characterization of a group of nucleoid-associated proteins displaying some properties shown by the histones, i.e. heat stability, acid solubility and abundance in the cell.

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References

  • Alberts B, Herrick G (1971) In: Colowick SP, Kaplan NO (eds) Methods in enzymology, vol 21D. Academic Press, New York, pp 198–217

    Google Scholar 

  • Bligh EG, Dyer MJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917

    Article  PubMed  CAS  Google Scholar 

  • Bohin JP, Bohin A, Schaeffer P (1976) Increased nitrate reductase A activity as a sign of membrane alteration in early blocked asporogenous mutants of Bacillus subtilis. Biochimie (Paris) 58:99–108

    CAS  Google Scholar 

  • Bowen B, Steinberg J, Laemmli UK, Weintraub H (1980) The detection of DNA-binding proteins by protein blotting. Nucleic Acids Res 8:1–18

    Article  PubMed  CAS  Google Scholar 

  • Charret R, Guillen N, Fleury AM, Le Hégarat H, Hirschbein L (1980) Organization of the isolated Bacillus subtilis nucleoid. Biol Cell 38:105–114

    CAS  Google Scholar 

  • Dijk J, White SW, Wilson KS, Appelt K (1983) On the DNA binding protein II from Bacillus stearothermophilus. J Biol Chem 258:4003–4006

    PubMed  CAS  Google Scholar 

  • Fleury-Guérout AM (1978) Caractérisation des protéines associées au nucléoide de Bacillus subtilis. Thèse de doctorate de 3ème cycle, Université Paris-Sud

    Google Scholar 

  • Griffith JD (1976) Visualization of procaryotic DNA in a regularly condensed chromatin-like fiber. Proc Natl Acad Sci USA 73:563–567

    Article  PubMed  CAS  Google Scholar 

  • Guillen N, Le Hégarat F, Fleury AM, Hirschbein L (1978) Folded chromosomes of vegetative Bacillus subtilis: composition and properties. Nucleic Acids Res 5:475–489

    Article  PubMed  CAS  Google Scholar 

  • Hendrickson WG, Kusano T, Yamaki H, Balakhrishnan R, King M, Murchie J, Schaechter M (1982) Binding of the origin of replication of Escherichia coli to the outer membrane. Cell 30:915–923

    Article  PubMed  CAS  Google Scholar 

  • Hirschbein L, Guillen N (1982) Characterization, assay and use of isolated bacterial nucleoids. Methods Biochem Anal 28:297–328

    Article  PubMed  CAS  Google Scholar 

  • Hobot JA, Villiger W, Escaig J, Maeder M, Ryter A, Kellenberger E (1985) Shape and fine structure of nucleoids observed on sections of ultrarapidly frozen and cryosubstituted bacteria. J Bacteriol 162:960–971

    PubMed  CAS  Google Scholar 

  • Hübscher U, Lutz H, Kornberg A (1980) Novel histone H2A-like protein of Escherichia coli. Proc Natl Acad Sci USA 77:5097–5101

    Article  PubMed  Google Scholar 

  • Huerre C, Le Hégarat JC (1979) Etude des clones de Bacillus subtilis resistants à la tétracycline obtenus par transformation avec des plasmides recombinés in vitro. Microbiologie 1979, (Edns du CNRS) Paris, p 179

    Google Scholar 

  • Imbert R, Bachinger H, Bickle TA (1982) Purification and characterization of a small DNA-binding protein, HB, from Bacillus globigii. Eur J Biochem 122:627–632

    Article  Google Scholar 

  • Kaback HR (1971) In: Jacob B (ed) Methods in enzymology, vol 22. Academic Press, New York, pp 99–120

    Google Scholar 

  • Kleppe K, Ovrebö S, Lossius I (1979) The bacterial nucleoid. J Gen Microbiol 112:1–13

    PubMed  CAS  Google Scholar 

  • Kusano T, Steinmeth D, Hendrickson WG, Murchie J, King M, Benson A, Schaechter M (1984) Direct evidence for specific binding of the replicative origin of the Escherichia coli chromosome to the membrane. J Bacgeriol 158:313–316

    CAS  Google Scholar 

  • Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685

    Article  PubMed  CAS  Google Scholar 

  • Lathe R, Buc H, Lecocq JP, Bautz EKF (1980) Prokaryotic histone-like protein interacting with RNA Polymerase. Proc Natl Acad Sci USA 77:3548–3552

    Article  PubMed  CAS  Google Scholar 

  • Leibowitz PJ, Schaechter M (1975) The attachment of the bacterial chromosome to the cell membrane. Int Rev Cytol 41:1–28

    Article  PubMed  CAS  Google Scholar 

  • Litman R (1968) A deoxyribonucleic acid polymerase from Micrococcus luteus (Micrococcus lysodeikticus) isolated on deoxyribonucleic acid-cellulose. J Biol Chem 243:6222–6233

    PubMed  CAS  Google Scholar 

  • Materman EC, Van Gool AP (1978) Compact Escherichia coli nucleoids in a highly supercoilsed conformation. J Bacterial 135:703–706

    CAS  Google Scholar 

  • Nakayama T, Kurogi Y, Irijura M (1981) Purification of Polypeptide P6 with a molecular weight of 6,000 from the vegetative chromosomes of Bacillus subtilis. J Bacteriol 145:953–957

    PubMed  CAS  Google Scholar 

  • Pettijohn DE, Hecht RM (1973) RNA molecules bound to the folded bacterial genome stabilize DNA folds and segregate domains of supercoiling. Cold Spring Harbor Symp Quant Biol 38: 31–34

    Google Scholar 

  • Portalier R, Worcel A (1976) Association of the folded chromosome with the cell envelope of E. coli: Characterization of the proteins at the DNA-membrane attachment site. Cell 8:245–255

    Article  PubMed  CAS  Google Scholar 

  • Rouvière-Yaniv J, Gros F (1975) Characterization of a novel, low-molecular-weight DNA-binding protein from E. coli. Proc Natl Acad Sci USA 72:3428–3432

    Article  PubMed  Google Scholar 

  • Rouvière-Yaniv J, Yaniv M, Germond JE (1979) Escherichia coli DNA-binding protein HU forms nucleosome-like structure with circular double stranded DNA. Cell 17:265–274

    Article  PubMed  Google Scholar 

  • Ryter A, Chang A (1975) Localization of transcribing genes in the bacterial cell by means of high resolution autoradiography. J Mol Biol 98:797–810

    Article  PubMed  CAS  Google Scholar 

  • Saito H, Muira K (1963) Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim Biophys Acta 72:619–629

    Article  PubMed  CAS  Google Scholar 

  • Salti V, Le Hégarat F, Hirschbein L (1985) Isolation and characterization of small heat-stable acid-soluble DNA-binding proteins from Bacillus subtilis nucleoids. J Gen Microbiol 131:581–590

    PubMed  CAS  Google Scholar 

  • Sargent MG, Bennett MF (1985) Amplification of a major membrane-bound DNA sequence of Bacillus subtilis. J Bacteriol 161:589–595

    PubMed  CAS  Google Scholar 

  • Schaeffer P, Ionesco H, Ryter A, Balassa G (1965) La sproulation de Bacillus subtilis, étude génétique et physiologique. Col Int CNRS 124:553–563

    Google Scholar 

  • Sjåstad K, Fadnes P, Kruger PG, Lossius I, Kleppe K (1982) Isolation, properties and nucleolytic degradation of chromatin from Escherichia coli. J Gen Microbiol 128:3037–3050

    PubMed  Google Scholar 

  • Spassky A, Rimsky S, Garreau H, Buc H (1984) H1a an E. coli DNA-binding protein which accumulates in stationary phase, strongly compacts DNA in vitro. Nucleic Acids Res 12:5321–5340

    Article  PubMed  CAS  Google Scholar 

  • Sueoka N, Hammers JM (1974) Isolation of DNA-membrane complex in Bacillus subtilis. Proc Natl Acad Sci USA 71:4787–4791

    Article  PubMed  CAS  Google Scholar 

  • Toyoda H, Yamaguchi K, Yoshikawa H (1978) Chromosome-membrane association in Bacillus subtilis IV. Further purification of DNA-membrane complex by using a combination of centrifugation and electrophoresis. J Bacteriol 136:799–802

    PubMed  CAS  Google Scholar 

  • Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from Polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76: 4350–4354

    Article  PubMed  CAS  Google Scholar 

  • Varshavsky AJ, Nedospasov SA, Bakayev VV, Bakayeva TG, Georgiev GP (1977) Histone-like proteins in the purified Escherichia coli deoxyribonucleoprotein. Nucleic Acids Res 4:2725–2745

    Article  PubMed  CAS  Google Scholar 

  • Worcel A, Burgi E (1972) On the structure of the folded chromosome of Escherichia coli. J Mol Biol 71:127–147

    Article  PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Institut de Microbiologie, Bat. 409, Université Paris-Sud, 91405, Orsay Cedex, France

    L. Hirschbein

Authors
  1. F. Le Hégarat
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  2. V. Salti
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  3. L. Hirschbein
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Editor information

Editors and Affiliations

  1. Department of Cell Biology, Laboratory of Genetics, University of Camerino, I-62032, Camerino (MC), Italy

    Claudio O. Gualerzi

  2. Max-Planck-Institut für Molekulare Genetik, Ihnestraße 73, D-1000, Berlin 33, Germany

    Claudio O. Gualerzi  & Cynthia L. Pon  & 

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© 1986 Springer-Verlag Berlin Heidelberg

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Hégarat, F.L., Salti, V., Hirschbein, L. (1986). Characterization of Bacillus subtilis Nucleoid DNA-Binding Proteins. In: Gualerzi, C.O., Pon, C.L. (eds) Bacterial Chromatin. Proceedings in Life Sciences. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-71266-1_12

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  • DOI: https://doi.org/10.1007/978-3-642-71266-1_12

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-71268-5

  • Online ISBN: 978-3-642-71266-1

  • eBook Packages: Springer Book Archive

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