Abstract
Protein-protein and protein-DNA interactions are of fundamental importance in the physical packaging of DNA in all Biological systems. In contrast to eukaryotes, where detailed information concerning the basic structural unit of chromatin (i.e. the nucleosome) is already available, studies on the mechanism responsible for the packaging of DNA in prokaryotes are still at an early stage. It is already clear, however, that new strategies must be devised when dealing with “bacterial chromatin” since no stable nucleosomelike structures can be obtained from bacterial cells. Furthermore, experiments with “intact” nucleoids are often thwarted by the difficulties intrinsic in work with an object of very large size and elusive chemical composition and physical properties (Hirshbein and Guillen 1982). Finally, the Biological organization of the bacterial cell probably requires very plastic and flexible structures for the packaging of DNA, rather than structures endowed with the structural rigidity of eukaryotic chromatin.
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© 1986 Springer-Verlag Berlin Heidelberg
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Paci, M., Pon, C.L., Canonaco, M.A., Gualerzi, C.O. (1986). Proteins from the Prokaryotic Nucleoid. [1H]-NMR Studies on Escherichia coli Histone-like Proteins NS and H-NS and Their Interaction with DNA. In: Gualerzi, C.O., Pon, C.L. (eds) Bacterial Chromatin. Proceedings in Life Sciences. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-71266-1_11
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DOI: https://doi.org/10.1007/978-3-642-71266-1_11
Publisher Name: Springer, Berlin, Heidelberg
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