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Secretion Cloning Vectors for Guiding the Localization of Proteins in Vivo

  • Conference paper
Book cover Protein Secretion and Export in Bacteria

Part of the book series: Current Topics in Microbiology and Immunology ((CT MICROBIOLOGY,volume 125))

Abstract

Cloning of genes into high copy number vectors has supplied biochemists with a powerful method for the production and purification of large amounts of biochemically important macromolecules. Judicious choice of promoter sequences has produced vectors capable of loading cells with 50% of the total cellular protein as the desired gene product, making purification a trivial chore. However, this approach is limited to proteins whose activity is not detrimental to protein production, and to proteins not sensitive to cytoplasmic proteases (see below). Recognition of this problem has stimulated the development of several cloning vectors designed to guide a cloned gene product out of the cytoplasm of the bacterial cell. These vectors produce a hybrid protein in which the cloned gene product is fused with a signal peptide, a structural element necessary for protein translocation. In this review, we will describe the advantages and limitations of some currently available secretion cloning vectors, and will discuss the use of these tools to produce large amounts of protein and to determine the information required to direct proteins to have various compartments of the cell.

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Author information

Authors and Affiliations

  1. Department of Biochemistry, State University of New York, Stony Brook, NY, 11794, USA

    C. A. Lunn & M. Inouye

  2. Biochemical Research Laboratory, Tokyo Jozo Co. Ltd., 632-1 Mifuku, Ohito-Cho, Tagata-Gun, Shizuoka-Ken, Japan, 410-23

    M. Takahara

Authors
  1. C. A. Lunn
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  2. M. Takahara
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  3. M. Inouye
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Editor information

Editors and Affiliations

  1. Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD, 20814-4799, USA

    Henry C. Wu M.D. Ph.D (Professor of Microbiology) (Professor of Microbiology)

  2. Department of Metabolic Regulation, Boston Biomedical Research Institute, 20 Staniford Street, Boston, MA, 02114, USA

    Phang C. Tai Ph.D (Senior Staff Scientist, Associate Professor) (Senior Staff Scientist, Associate Professor)

  3. Department of Microbiology and Molecular Genetics, Harvard Medical School, 23 Shattuck Street, Boston, MA, 02115, USA

    Phang C. Tai Ph.D (Senior Staff Scientist, Associate Professor) (Senior Staff Scientist, Associate Professor)

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© 1986 Springer-Verlag Berlin · Heidelberg

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Lunn, C.A., Takahara, M., Inouye, M. (1986). Secretion Cloning Vectors for Guiding the Localization of Proteins in Vivo. In: Wu, H.C., Tai, P.C. (eds) Protein Secretion and Export in Bacteria. Current Topics in Microbiology and Immunology, vol 125. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-71251-7_6

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  • DOI: https://doi.org/10.1007/978-3-642-71251-7_6

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-71253-1

  • Online ISBN: 978-3-642-71251-7

  • eBook Packages: Springer Book Archive

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