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Retardation of Spermiation Following Short-Term Treatment of Rats with Theobromine

  • R. A. Ettlin
  • J. M. Armstrong
  • S. Buser
  • U. Hennes
Part of the Archives of Toxicology book series (TOXICOLOGY, volume 9)

Abstract

Groups of 4–6 Fü-albino rats were examined 24 h after 3 daily doses of 500 mg/kg bw of theobromine administered orally by gavage as well as 1, 2,4, 6, and 10 weeks after 5 daily doses. Controls received the standard solvent vehicle (SSV) only. A variety of parameters were assessed including body and organ weights, serum clinical chemistry, hematological parameters. epididy­mal sperm motility and LDH-X fraction in seminal plasma, serum gonadotro­pins and testosterone, and the morphology of various organs. Testes were perfused with 5% glutaraldehyde and semi-thin sections were evaluated. The most striking morphological observation was a retarded release of late sper­matids into the tubular lumen mainly 2 weeks post treatment. This partial dis­ruption of the rigid spermatogenic synchronization was not followed by sub­stantial germ cell death. The other parameters investigated remained rela­tively normal throughout the study. These observations suggest that theobro­mine at the dose tested rather selectively interferes with germ cell kinetics. Ser­toli cell toxicity could account for these early and subtle effects as well as for the late and severe effects of subchronic exposure of rats to theobromine as reported in the literature.

Key words

Testicular toxicity Spermatogenic desynchronization Sertoli cells LDH-X Theobromine Rats 

Abbreviations

Th

theobromine

SC

Sertoli cell

SSV

standard solvent vehicle (containing carboxy­methylcellulose and Tween 70)

LDH

lactate dehydrogenase

LDH-X

fraction X of LDH (different isoenzymes)

RBC

red blood cells count

Hb

hemoglobin

MCV

mean corpuscular volume

PCV

packed cell volume

MCH

mean corpuscular hemoglobin

MCHC

mean corpuscular hemoglobin con­centration

WBC

white blood cell count

GOT

GPT transaminases

AP

alkaline phosphatase

SR

acid phosphatase

LH

luteinizing hormone

FSH

follicle stimulating hormone

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Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • R. A. Ettlin
    • 1
  • J. M. Armstrong
    • 2
  • S. Buser
    • 1
  • U. Hennes
    • 1
  1. 1.F. Hoffmann-La Roche & Co. Ltd.BaselSwitzerland
  2. 2.Hoffmann-La Roche Inc.NutleyUSA

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