Monooxygenase Activity of Fish Liver in Biomonitoring Aquatic Environment
The hepatic biotransformation activity of four cultivated freshwater fish, Amur carp (Ctenopharyngodon idella), carp (Cyprinus carpio), rainbow trout (Salmo gairdneri) and tench (Tinca tinca) were studied one day after catching the fish from ponds and a river in the south of GDR and in fish kept for two weeks in clean filtrated and deacidified water. Aryl hydrocarbon hydroxylase, 7-ethoxycoumarin O-deethylase and 7-ethoxyresorufin O-deethylase activities fell to about 20% during the stay in clean water in rainbow trout liver microsomes. The reductions were smaller in the other species studied.
Key wordsMonooxygenase Fish liver Water pollution
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- Burke MD, Mayer RT (1974) Ethoxyresorufin: direct fluorimetric assay of a microsomal O-dealkylation which is preferentially inducible by 3-methylcholanthrene. Drug Metab Disp 2: 583–588Google Scholar
- DeWaide JH (1971) Metabolism of xenobiotics. Comparative and kinetic studies as a basis for environ-mental pharmacology. Thesis, University of Nijmegen, NetherlandsGoogle Scholar
- Koivusaari U, Lindström-Seppä P, Lang M, Harri M, Hänninen O (1980) Occurrence of cytochrome P-450 in certain fresh water species in Northern Europe. In: Gustafsson J-A (ed) Biochemistry, Biophysics, and Regulation of Cytochrome P-450. Elsevier/North-Holland Biomedical Press, Netherlands, pp 455–458Google Scholar
- Laitinen M, Hietanen E, Nieminen M, Pasanen P (1984) Acidification of water and extrahepatic bio-transformation in fish. Environ Pollut 35A: 271–278Google Scholar
- Payne JF (1984) Mixed-function oxygenases in biological monitoring programs: Review of potential usage in different phyla of aquatic animals. In: Persoone G, Jaspers E, Claus C (eds) Proceedings of International Symposium on Ecotoxicological Testing for Marine Environment. Ghent, BelgiumGoogle Scholar
- Pickering QH, Hunt EP, Phipps GL, Roush TH, Smith WE, Spehar DL, Stephan CE, Tanner DK (1983) Effects of pollution on freshwater fish and amphibians. J Water Pollut Control Fed 55: 840–863Google Scholar