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Effects of Zineb on Hepatic Microsomal Systems in Rats and Mice

  • H. Michalek
  • A. Meneguz
Conference paper
Part of the Archives of Toxicology book series (TOXICOLOGY, volume 9)

Abstract

The effects of acute oral treatment with Zineb on hepatic microsomal xenobiotic metabolizing systems were studied in rats and mice. Administration of Zineb at doses from 200 to 600 mg/kg caused a significant impairment of aminopyrine-N-demethylase in the two species, as well as an inhibition of aniline hydroxylase in rats and a slight increase of this enzymatic activity in mice. The effects were absent at 2 h, reached maximum at 24 h and disappeared within 48 h after treatment. The cytochrome P-450 levels were not modified. The inhibitory and stimulatory effects of Zineb were discussed in relation to its metabolites such as ethylenethiourea and ethylenebisdithiocynato sulfide in these animal species.

Key words

Zineb Ethylenebisdithiocarbamate fungicides Mixed function oxidase Cytochrome P-450 

References

  1. Albrecht R, Pélissier MA, Manchon P, Dupuis D (1975) Influence de l’administration de parathion-méthyle ou de zinèbe sur l’activité de quelques enzymes hépatiques chez le rat. Ann Nutr Alim 29: 223–238Google Scholar
  2. Jordan LW, Neal RA (1979) Examination of the in vivo metabolism of maneb and zineb to ethylenthiourea ( ETU) in mice. Bull Environ Contam Toxicol 22: 271–277PubMedCrossRefGoogle Scholar
  3. Lewerenz HJ, Plass R (1984) Contrasting effects of ethylenethiourea on hepatic monooxygenases in rats and mice. Arch Toxicol 56: 92–95PubMedCrossRefGoogle Scholar
  4. Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265–275PubMedGoogle Scholar
  5. Mazel P (1971) Experiments illustrating drug metabolism in vitro. In: La Du BN, Mandel MG, Way EL (eds) Fundamentals of drug metabolism and drug disposition. Williams and Wilkins, BaltimoreGoogle Scholar
  6. Smith RB, Finnegan JK, Larson PS, Sahyoun PF, Dreyfuss ML, Haag HB (1953) Toxicologic studies on zinc and disodium ethylenebisdithiocarbamates. J Pharmacol Exp Ther 109: 159–166PubMedGoogle Scholar
  7. Yoshida T, Jordan L, Neal RA (1978) Effects of ethylenebisdiisothiocyanato sulfide (EBIS) on hepatic monooxygenase activity. Toxicol Appl Pharmacol 46: 215–225PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • H. Michalek
    • 1
  • A. Meneguz
    • 1
  1. 1.Laboratory of PharmacologyIstituto Superiore di SanitàRomaItaly

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