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Mushroom Poisoning due to Cortinarius Speciosissimus: Electron Microscope Study in Rats

  • S. Lahtiperä
  • A. Naukkarinen
  • Y. Collan
Part of the Archives of Toxicology book series (TOXICOLOGY, volume 9)

Abstract

The effects of a poisonous mushroom, Cortinarius speciosissimus, on rat kidney were studied by transmission electron microscopy. Suspension of dried mushroom (500 mg/kg body weight) was administered as a single dose directly into the esophagus. The kidneys were fixed by perfusion, and both cortex and medulla were sampled. C. speciosissimus toxin acted primarily on the epithelial cells of the proximal tubules. No changes were seen in the glomeruli. First ultrastructural changes were observed at 2 days in the renal cortex. The most prominent damage occurred at 5 days, when most of the proximal tubular cells appeared necrotic. Dark bodies (diameter 0.15–0.5 µm) spaced by 25–45 nm were frequently found in the damaged tubular cell nuclei. Regeneration of the tubular cells was seen at 10 days. After 2 months, increased amount of collagen fibres were seen between tubules.

The nuclear changes in the damaged tubular cells and the slowly manifesting toxicity suggest that C. speciosissimus toxin acts on nuclei or nucleoli and/or metabolic pathways associated with them.

Key words

Nephrotoxicity Mushroom poisoning Cortinarius speciosissimus Nuclear bodies Electron microscopy 

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References

  1. Antkowiak W, Gessner W (1979) The structures of orellanine and orelline. Tetrahedron letters 21: 1931–1934CrossRefGoogle Scholar
  2. Bohman S (1974) The ultrastructure of the rat renal medulla as observed after improved fixation methods. J Ultrastruct Res 47: 329–360PubMedCrossRefGoogle Scholar
  3. Grzymala S (1965) Etude clinique des intoxications par les champignons de genre Cortinarius orellanus. Fr Bull Med leg toxicol 8: 60–70Google Scholar
  4. Hulmi S, Sipponen P, Forsström J, Vilska J (1974) Mushroom poisoning caused by Cortinarius speciosissimus. A report of four cases. Duodecim 90: 1044–1050 (in Finnish)Google Scholar
  5. Larsson L (1974) Effects of different fixatives on the ultrastructure of the developing proximal tubule in the rat kidney. J Ultrastruct Res 51: 140–151CrossRefGoogle Scholar
  6. Litten W (1975) The most poisonous mushrooms. Sci Am 3: 90–101CrossRefGoogle Scholar
  7. Nieminen L, Möttönen M, Tirri R, Ikonen S (1975) Nephrotoxicity of Cortinarius speciosissimus: A histological and enzyme histochemical study. Exp Pathol Bd 11: 239–246Google Scholar
  8. Trump B, Arstila A (1975) Cell injury and cell death. In: LaVia M, Hill R (eds) Principles of pathobiology, pp 9–96. Oxford University Press, New York, London, TorontoGoogle Scholar

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • S. Lahtiperä
    • 1
  • A. Naukkarinen
    • 1
  • Y. Collan
    • 1
  1. 1.Department of PathologyUniversity of KuopioKuopioFinland

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