Abstract
Patulin is a mycotoxin produced by various Penicillium, Aspergillus and Byssochlamys species. To evaluate its inhibitory effect on cells, hepatoma tissue culture cells in suspension were incubated in presence of 30 μM of patulin for 7 h and investigated by transmission and scanning electron microscopy. By transmission electron microscopy, the most significant difference observed between treated and control cells was the disorganization of the cytoplasmic microfilaments in the treated cells. The disappearance of superficial membrane microvilli which contain microfibrillar material was visualized by scanning electron microscopy; the cells also presented protrusions. The effect of this toxin on the cytoskeleton can be compared to that exerted by colchicine or by cytochalasines.
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References
Ciegler A, Vesonder RF, Jackson LK (1977) Production and biological activity of patulin and citrinin from Penicillium expansum. Appl Environ Microbiol 33: 1004–1006
Hatey F, Gaye P (1978) Inhibition of translation in reticulocyte lysate by the mycotoxin patulin. FEBS Lett 95: 252–256
Hatey F, Moulé Y (1979) Protein synthesis inhibition in rat liver by the mycotoxin patulin. Toxicology 13: 223–231
Hayes AW, Phillips TD, Williams WL, Ciegler A (1979) Acute toxicity of patulin in mice and rats. Toxicology 13: 91–100
Korte A (1980) Chromosomal analysis in bone-marrow cells of chinese hamster after treatment with mycotoxins. Mut Res 78: 41–49
McKinley ER, Carlton WW (1980) Patulin mycotoxicosis in the Syrian hamster. Fd Cosmet Toxicol 18: 173–179
Moulé Y, Hatey F (1977) Mechanism of the in vitro inhibition of transcription by patulin, a mycotoxin from Byssochlamys nivea. FEBS Lett 74: 121–125
Phillips TD, Hayes AW (1979) Inhibition of electrogenic sodium transport across toad urinary bladder by the mycotoxin patulin. Toxicology 13: 17–24
Reddy CS, Chan PK, Hayes AW, Williams WL (1979) Acute toxicity of patulin and its interaction with penicillic acid in dogs. Fd Cosmet Toxicol 17: 605–609
Rihn B, Dirheimer G, Lugnier AAJ (1982) Effect of patulin on macromolecular synthesis on HTC cells. In: Mycotoxins and Phycotoxins. Technical University Vienna ed (1982) pp 317–320
Schaeffer WI, Smith NE, Payne PA (1975) Physiological and biochemical effects of the mycotoxin patulin on Chang liver cell cultures. In vitro II: 69–77
Scott PM (1974) Patulin. In: Purchase IFH (ed) Mycotoxins. Elsevier, Amsterdam, pp 383–403
Singh J (1967) Patulin. In: Gottlieb D, Shaw PH (eds) Antibiotics: Mechanism of action, vol 1. Springer-Verlag, Berlin Heidelberg New York, pp 621–630
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© 1986 Springer-Verlag
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Rihn, B., Lugnier, A.A.J., Dirheimer, G. (1986). Morphological Alterations Induced by Patulin on Cultured Hepatoma Cells. In: Chambers, C.M., Chambers, P.L., Tuomisto, J. (eds) Toxic Interfaces of Neurones, Smoke and Genes. Archives of Toxicology, vol 9. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-71248-7_43
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DOI: https://doi.org/10.1007/978-3-642-71248-7_43
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-16589-7
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