Significance of T4 or T8 Phenotype of Human Cytotoxic T-Lymphocyte Clones
Mature human T lymphocytes can be divided into two mutually exclusive sub-populations by monoclonal antibodies (mAb) to the T4 and T8 markers. On the basis of cell separation experiments, the helper function forBor T lymphocytes, the proliferative capacity to soluble antigens (tuberculin, tetanus toxoid) and to alloantigens has been assigned to the T4/Leu 3 subset, while cytotoxic and suppressor functions are associated with the T8/Leu 2 subset (Reinherz and Schlossman 1980; Engleman et al. 1981). Furthermore, T4+and T8+T-cell populations exhibit different specificity for either HLA class II or class I antigens, both as restriction elements or as target alloantigens. Improved culture conditions and the use of interleukin 2 (IL-2) as T-cell growth factor made it possible to clone human T lymphocytes, enabling cloned T-cell populations to be used to analyze functions and specificities of various T-cell subsets at the single- cell level.
KeywordsInfectious Mononucleosis Cytotoxic Function Improve Culture Condition Cell Separation Experiment Primary Mixed Lymphocyte Culture
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