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Significance of T4 or T8 Phenotype of Human Cytotoxic T-Lymphocyte Clones

  • B. Fleischer
  • H. Wagner
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 126)

Abstract

Mature human T lymphocytes can be divided into two mutually exclusive sub-populations by monoclonal antibodies (mAb) to the T4 and T8 markers. On the basis of cell separation experiments, the helper function forBor T lymphocytes, the proliferative capacity to soluble antigens (tuberculin, tetanus toxoid) and to alloantigens has been assigned to the T4/Leu 3 subset, while cytotoxic and suppressor functions are associated with the T8/Leu 2 subset (Reinherz and Schlossman 1980; Engleman et al. 1981). Furthermore, T4+and T8+T-cell populations exhibit different specificity for either HLA class II or class I antigens, both as restriction elements or as target alloantigens. Improved culture conditions and the use of interleukin 2 (IL-2) as T-cell growth factor made it possible to clone human T lymphocytes, enabling cloned T-cell populations to be used to analyze functions and specificities of various T-cell subsets at the single- cell level.

Keywords

Infectious Mononucleosis Cytotoxic Function Improve Culture Condition Cell Separation Experiment Primary Mixed Lymphocyte Culture 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1986

Authors and Affiliations

  • B. Fleischer
    • 1
  • H. Wagner
    • 1
  1. 1.Department of Medical Microbiology and ImmunologyUniversity of UlmUlmGermany

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