Forensic Applications of Human Body Fluid Esterases
Esterases of human semen and vaginal secretion show different electrophoretic patterns when studied using polyacrylamide gel with a thin strip of starch gel at the origin. Vaginal esterase occurs as a single cathodal zone, while semen esterase is manifested as a number of cathodal bands and hence the method can be used to distinguish semen and vaginal secretion in sexual assault cases.
Under these conditions, pseudocholinesterase (PCE) from blood plasma is detected as anodal zones and hence a mixture of blood and vaginal secretion can be demonstrated, indicating, but not proving that a bloodstain is of menstrual origin.
Experiences with the technique in the examination of a range of vaginal swabs is presented.
KeywordsStarch Hydroxide Propionate Electrophoresis Sodium Hydroxide
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