Forensic Applications of Human Body Fluid Esterases

  • Brian H. Parkin
Conference paper
Part of the Advances in Forensic Haemogenetics book series (HAEMOGENETICS, volume 1)


Esterases of human semen and vaginal secretion show different electrophoretic patterns when studied using polyacrylamide gel with a thin strip of starch gel at the origin. Vaginal esterase occurs as a single cathodal zone, while semen esterase is manifested as a number of cathodal bands and hence the method can be used to distinguish semen and vaginal secretion in sexual assault cases.

Under these conditions, pseudocholinesterase (PCE) from blood plasma is detect­ed as anodal zones and hence a mixture of blood and vaginal secretion can be demonstrated, indicating, but not proving that a bloodstain is of menstrual origin.

Experiences with the technique in the examination of a range of vaginal swabs is presented.


Esterase Activity Thin Strip Menstrual Blood Vaginal Swab Vaginal Fluid 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1986

Authors and Affiliations

  • Brian H. Parkin
    • 1
  1. 1.The Metropolitan Police Forensic Science LaboratoryLondonUK

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