An ELISA Method for Detecting Lewis Antigens in Body Fluids
Detection of Lewis a (Lea) or Lewis b (Le b) antigens in body fluids enables the prediction or corroboration of the ability of the fluid depositor to secrete soluble ABH substances (1–4). The hemagglutination-inhibition technique, usually employed for the detection of the Lewis antigens, is not well suited for processing large numbers of samples on a routine basis. The genesis of monoclonal antibodies, specific for Le a and Le b, has promoted the development of enzyme-linked immunosorbent assays (ELISA) for the detection of these antigens (5–6). The ELISA described in this paper incorporates these monoclonal reagents into a technique that can be used to efficiently analyze a large number of samples for the presence of Le a and Le b in body fluids.
KeywordsGlycine Immobilization bOiling Polystyrene Gelatin
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