Electrophoretic “Subtyping” of Rare ESD Variants

  • W. Bär
  • V. Biedermann
Conference paper
Part of the Advances in Forensic Haemogenetics book series (HAEMOGENETICS, volume 1)


The common allozymes 1,2 and 5 of red cell esterase D can be determined in a clear-cut manner by agarose gel electrophoresis using a continuous buffer system at pH 5.35.–5.45 composed of malic acid (1). At acidic pH values the allozyme 5 migrates definitely more anodically than the allozyme 2; at neutral pH this distinction is ambiguous. With this new electrophoretic method, isoelectric focusing of ESD 2–1 and 2 is not necessary anymore to detect the gene product.of ESD 5. To show the usefulness of the electrophoretic method to detect rare variants was the aim of this investigation.


Malic Acid Rare Variant Forensic Medicine Redistilled Water Electrophoretic Method 
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  1. 1).
    Bär W, Häni M, Biedermann V (1984) Esterase D: Simultaneous electrophoretic determination of the three common allozymes (ESD 1,2,5). Electrophoresis 5: 280–281CrossRefGoogle Scholar
  2. 2).
    Nishigaki I, Itoh T (1984) Isoelectric focusing studies of human red cell esterase D: Evidence for polymorphic occurrence of a new allele ESD7 in Japanese. Hum Genet 66: 92–95PubMedCrossRefGoogle Scholar
  3. 3).
    Henke J, Schweitzer H, Bär W (1985) Elektrophoretische Darstellung der Produkte der Esterase-D-Allele ESD*1,ESD*2,ESD*5 and ESD*7. Aerztl Lab 31: 123–124Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1986

Authors and Affiliations

  • W. Bär
    • 1
  • V. Biedermann
    • 1
  1. 1.Institute of Forensic MedicineUniversity of ZürichSwitzerland

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