Electrophoretic “Subtyping” of Rare ESD Variants

Abstract

The common allozymes 1,2 and 5 of red cell esterase D can be determined in a clear-cut manner by agarose gel electrophoresis using a continuous buffer system at pH 5.35.–5.45 composed of malic acid (1). At acidic pH values the allozyme 5 migrates definitely more anodically than the allozyme 2; at neutral pH this distinction is ambiguous. With this new electrophoretic method, isoelectric focusing of ESD 2–1 and 2 is not necessary anymore to detect the gene product.of ESD 5. To show the usefulness of the electrophoretic method to detect rare variants was the aim of this investigation.