Advertisement

Alpha-l-Antitrypsine (Pi) Polymorphism. Improved Pi-M-Subtyping by PAGIF with Immobilized Gradients.

  • F. Ferstl
  • H. H. Hoppe
Conference paper
Part of the Advances in Forensic Haemogenetics book series (HAEMOGENETICS, volume 1)

Abstract

The demonstration of the genetically determined alpha-l-antitrypsine (PI) phenotypes may be achieved generally by isoelectrofocusing (IEF) on polyacrylamide gels (PAGIF) with carrier ampholytes. This method which originally was applied at a pH-gradient pH 3.5 — pH 6.does not produce a sufficient resolution quality though recently carrier ampholytes with a pH gradient pH 4.2 – 4.9 are offering an improved resolution (3).

Keywords

Sulfosalicylic Acid Improve Resolution Sample Appli Carrier Ampholyte Stabile Buffer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Lit.

  1. (1).
    Fawcett, J.S. (1975) in Isoelectric Focusing and Isotachophoresis (Righetti, P.G. ed.), pp. 25–37 North Holland/American Elsevier, AmsterdamGoogle Scholar
  2. (2).
    Rilbe, H. (1977) in Electrofocusing and Isotachophoresis (Radola, B. J. and Graesslin,D., eds.), pp. 35–50 de Gruyter, BerlinGoogle Scholar
  3. (3).
    Martin, W.: Persönliche MitteilungGoogle Scholar
  4. (4).
    Görg, A., Postel, W., Westermeier,R., Gianazza, E., Righetti, P.G.: Biochem. Biophys. Methods 3 (1980) 273–284CrossRefGoogle Scholar
  5. (5).
    Constans, J., Viau, M., Gouaillard, C. (1980) Human. Genet. 55: 119–121CrossRefGoogle Scholar
  6. (6).
    Ferstl, F.: Inaugural Dissertation (in press)Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1986

Authors and Affiliations

  • F. Ferstl
    • 1
  • H. H. Hoppe
    • 1
  1. 1.Zentralinstitut für TransfusionsmedizinHamburg Filbektal 111Germany

Personalised recommendations