Alpha-l-Antitrypsine (Pi) Polymorphism. Improved Pi-M-Subtyping by PAGIF with Immobilized Gradients.

  • F. Ferstl
  • H. H. Hoppe
Conference paper
Part of the Advances in Forensic Haemogenetics book series (HAEMOGENETICS, volume 1)


The demonstration of the genetically determined alpha-l-antitrypsine (PI) phenotypes may be achieved generally by isoelectrofocusing (IEF) on polyacrylamide gels (PAGIF) with carrier ampholytes. This method which originally was applied at a pH-gradient pH 3.5 — pH 6.does not produce a sufficient resolution quality though recently carrier ampholytes with a pH gradient pH 4.2 – 4.9 are offering an improved resolution (3).


Sulfosalicylic Acid Improve Resolution Sample Appli Carrier Ampholyte Stabile Buffer 
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  1. (1).
    Fawcett, J.S. (1975) in Isoelectric Focusing and Isotachophoresis (Righetti, P.G. ed.), pp. 25–37 North Holland/American Elsevier, AmsterdamGoogle Scholar
  2. (2).
    Rilbe, H. (1977) in Electrofocusing and Isotachophoresis (Radola, B. J. and Graesslin,D., eds.), pp. 35–50 de Gruyter, BerlinGoogle Scholar
  3. (3).
    Martin, W.: Persönliche MitteilungGoogle Scholar
  4. (4).
    Görg, A., Postel, W., Westermeier,R., Gianazza, E., Righetti, P.G.: Biochem. Biophys. Methods 3 (1980) 273–284CrossRefGoogle Scholar
  5. (5).
    Constans, J., Viau, M., Gouaillard, C. (1980) Human. Genet. 55: 119–121CrossRefGoogle Scholar
  6. (6).
    Ferstl, F.: Inaugural Dissertation (in press)Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1986

Authors and Affiliations

  • F. Ferstl
    • 1
  • H. H. Hoppe
    • 1
  1. 1.Zentralinstitut für TransfusionsmedizinHamburg Filbektal 111Germany

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