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The Use of Immunological Techniques in Plant Hormone Analysis

  • Conference paper
Plant Growth Substances 1985

Part of the book series: Proceedings in Life Sciences ((LIFE SCIENCES))

Abstract

Immunoassays were first used by Berson and Yalow [4] to measure insulin in human blood and the technique was subsequently adapted for analysis of a variety of compounds involved in human and animal metabolism [1]. Antisera raised against indole-3-acetic acid (IAA) and gibberellin A3 were initially used in an assay based on inhibition of activation of modified bacteriophage T4 [6, 8, 9]. However, these reports received only limited attention and it was not until the development of radioimmunoassays (RIA) [2, 10, 14, 18–21, 25] and enzyme-linked immunoabsorbent assays (ELISA [3, 5, 22, 24] for plant hormones that the potential value of immunological techniques for growth regulator research began to be more generally appreciated.

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References

  1. Abraham GE (1977) Handbook of radioimmunoassay. Dekker, New York

    Google Scholar 

  2. Atzhorn R. Weiler EW (1938a) Planta (Berl) 159:1

    Article  Google Scholar 

  3. Atzorn R, Weiler EW (1983b) Planta (Berl) 159:7

    Article  CAS  Google Scholar 

  4. Bersen SA, Yalow RS (1959) Ann NY Acad Sci 82:338

    Article  Google Scholar 

  5. Daie J, Wyse R (1982) Anal Biochem 119:365

    Article  PubMed  CAS  Google Scholar 

  6. Fuchs S, Fuchs Y (1969) Biochim Biophys Acta 192:528

    Article  PubMed  CAS  Google Scholar 

  7. Fuchs Y, Gertman E (1974) Plant Cell Physiol 15:629

    CAS  Google Scholar 

  8. Fuchs S, Haimovich J, Fuchs Y (1971) Eur J Biochim 18:384

    Article  CAS  Google Scholar 

  9. Fuchs Y, Mayak S, Fuchs S (1972) Planta (Berl) 103:117

    Article  CAS  Google Scholar 

  10. Khan SA, Humayun MZ, Jacob TM (1977) Anal Biochem 83:632

    Article  PubMed  CAS  Google Scholar 

  11. MacDonald EMS, Morris RO (1985) Methods Enzymol 110:347

    Article  CAS  Google Scholar 

  12. Mertens R, Stüning M, Weiler EW (1982) Naturwissenschaften 69:595

    Article  CAS  Google Scholar 

  13. Morris RO, Akiyoshi DE, MacDonald EMS, Morris JW, Regier DA, Zaerr JD (1982) In Wareing PF (ed) Plant growth substances 1982. Academic Press, London, p 175

    Google Scholar 

  14. Pengelly WL, Meins F Jr (1977) Planta (Berl) 136:173

    Article  CAS  Google Scholar 

  15. Reeve DR, Crozier A (1980) (ed) Encyclopedia of plant physiology, new series, vol 9. Hormonal regulation of development I. Molecular aspects of plant hormones. Springer, Berlin Heidelberg New York, p 203

    Google Scholar 

  16. Sandberg G, Ljung K, Alm P (1985) Phytochemistry (Oxf) 24:1439

    Article  CAS  Google Scholar 

  17. Sundberg B, Sandberg G, Crozier A (1986) Pytochemistry (Oxf) 25:295

    Article  CAS  Google Scholar 

  18. Walton D, Dashek W, Galson E (1979) Planta (Berl) 146:139

    Article  CAS  Google Scholar 

  19. Weiler EW (1979) Planta (Berl) 144:255

    Article  CAS  Google Scholar 

  20. Weiler EW (1980) Planta (Berl) 149:155

    Article  CAS  Google Scholar 

  21. Weiler EW (1981) Planta (Berl) 153:319

    Article  CAS  Google Scholar 

  22. Weiler EW (1982) Physiol Plant 54:510

    Article  CAS  Google Scholar 

  23. Weiler EW (1984) Annu Rev Plant Physiol 35:85

    Article  CAS  Google Scholar 

  24. Weiler EW, Jourdan PS, Conrad W (1981) Planta (Berl) 153:561

    Article  CAS  Google Scholar 

  25. Weiler EW, Wieczorek U (1981) Planta (Berl) 152:159

    Article  CAS  Google Scholar 

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© 1986 Springer-Verlag Berlin Heidelberg

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Crozier, A., Sandberg, G., Monteiro, A.M., Sundberg, B. (1986). The Use of Immunological Techniques in Plant Hormone Analysis. In: Bopp, M. (eds) Plant Growth Substances 1985. Proceedings in Life Sciences. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-71018-6_2

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  • DOI: https://doi.org/10.1007/978-3-642-71018-6_2

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-71020-9

  • Online ISBN: 978-3-642-71018-6

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