The Use of Immunological Techniques in Plant Hormone Analysis

  • A. Crozier
  • G. Sandberg
  • A. M. Monteiro
  • B. Sundberg
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)


Immunoassays were first used by Berson and Yalow [4] to measure insulin in human blood and the technique was subsequently adapted for analysis of a variety of compounds involved in human and animal metabolism [1]. Antisera raised against indole-3-acetic acid (IAA) and gibberellin A3 were initially used in an assay based on inhibition of activation of modified bacteriophage T4 [6, 8, 9]. However, these reports received only limited attention and it was not until the development of radioimmunoassays (RIA) [2, 10, 14, 18–21, 25] and enzyme-linked immunoabsorbent assays (ELISA [3, 5, 22, 24] for plant hormones that the potential value of immunological techniques for growth regulator research began to be more generally appreciated.


High Performance Liquid Chromatography High Performance Liquid Chromatography Quantification Point Immunoaffinity Chromatography Immunological Technique 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag Berlin Heidelberg 1986

Authors and Affiliations

  • A. Crozier
    • 1
  • G. Sandberg
    • 2
  • A. M. Monteiro
    • 1
    • 3
  • B. Sundberg
    • 2
  1. 1.Department of BotanyThe University of GlasgowGlasgowGreat Britain
  2. 2.Department of Forest Genetics and Plant PhysiologyThe Swedish University of Agricultural SciencesUmeaSweden
  3. 3.Department of Plant PhysiologyUNICAMPCampinasBrazil

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