The Use of Immunological Techniques in Plant Hormone Analysis

  • A. Crozier
  • G. Sandberg
  • A. M. Monteiro
  • B. Sundberg
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)

Abstract

Immunoassays were first used by Berson and Yalow [4] to measure insulin in human blood and the technique was subsequently adapted for analysis of a variety of compounds involved in human and animal metabolism [1]. Antisera raised against indole-3-acetic acid (IAA) and gibberellin A3 were initially used in an assay based on inhibition of activation of modified bacteriophage T4 [6, 8, 9]. However, these reports received only limited attention and it was not until the development of radioimmunoassays (RIA) [2, 10, 14, 18–21, 25] and enzyme-linked immunoabsorbent assays (ELISA [3, 5, 22, 24] for plant hormones that the potential value of immunological techniques for growth regulator research began to be more generally appreciated.

Keywords

High Performance Liquid Chromatography Maize Fluori Germinate MeOH 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1986

Authors and Affiliations

  • A. Crozier
    • 1
  • G. Sandberg
    • 2
  • A. M. Monteiro
    • 1
    • 3
  • B. Sundberg
    • 2
  1. 1.Department of BotanyThe University of GlasgowGlasgowGreat Britain
  2. 2.Department of Forest Genetics and Plant PhysiologyThe Swedish University of Agricultural SciencesUmeaSweden
  3. 3.Department of Plant PhysiologyUNICAMPCampinasBrazil

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