The Use of Immunological Techniques in Plant Hormone Analysis
Immunoassays were first used by Berson and Yalow  to measure insulin in human blood and the technique was subsequently adapted for analysis of a variety of compounds involved in human and animal metabolism . Antisera raised against indole-3-acetic acid (IAA) and gibberellin A3 were initially used in an assay based on inhibition of activation of modified bacteriophage T4 [6, 8, 9]. However, these reports received only limited attention and it was not until the development of radioimmunoassays (RIA) [2, 10, 14, 18–21, 25] and enzyme-linked immunoabsorbent assays (ELISA [3, 5, 22, 24] for plant hormones that the potential value of immunological techniques for growth regulator research began to be more generally appreciated.
KeywordsHigh Performance Liquid Chromatography High Performance Liquid Chromatography Quantification Point Immunoaffinity Chromatography Immunological Technique
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