Cellular Localization of Lipoprotein Receptors in Liver
Investigations on the quantitative role of the various cell types (parenchymal, Kupffer and endothelial cells) to the receptor-mediated uptake by the liver in vivo, were until recently hampered by the applied cell isolation procedures during which bound and/or internalized substances were processed and lost from the cells. The procedure as outlined in scheme 1 prevents such a processing because both liver perfusion, cell separation and cell purification are performed at a low temperature (at 8° C). In addition one is able to assess the recovery by comparing the radioactivity in the purified parenchymal, endothelial and Kupffer cells (all obtained from one liver) to the radioactivity originally present in the whole rat liver. The method was evaluated using125 I-asialofetuin as the substrate (Fig. 1) and compared to quantitative autoradiographic data .
KeywordsParenchymal Cell Kupffer Cell Lipoprotein Receptor Nylon Gauze Liver Endothelial Cell
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
Hubbard AL, Wilson G, Ashwell G, Stukenbrok H (1979) J Cell Biol 83: 47–64PubMedCrossRefGoogle Scholar
Harkes L, Van Berkel TJC (1984) Biochem J 224: 21–27PubMedGoogle Scholar
Goldstein JL, Ho YK, Basu SK, Brown MS (1979) Proc Natl Acad Sci USA 76: 333–337PubMedCrossRefGoogle Scholar
Henriksen T, Mahoney EM, Steinberg D (1981) Proc Natl Acad Sci USA 78: 6499–6503PubMedCrossRefGoogle Scholar
Van Berkel TJC, Nagelkerke JF, Harkes L, Kruijt JK (1982) Biochem J 208: 493–503PubMedGoogle Scholar
Nagelkerke JF, Barto KP, Van Berkel TJC (1983) J Biol Chem 258: 12221–12227PubMedGoogle Scholar
Mommaas-Kienhuis AM, Nagelkerke JF, Vermeer BJ, Daems WT, Van Berkel TJC (1985) Eur J Cell Biol 38: 42–50PubMedGoogle Scholar
Kempen HJM, Spanjer HH, Van Berkel THJC (1985) Eur J Cell Biol in pressGoogle Scholar
Van Berkel TJC (1982) in „Metabolic Compartmentation“ (H. Sies, ed) Academic Press Inc London pp 437–482Google Scholar
© Springer-Verlag Berlin Heidelberg 1986