Abstract
Investigations on the quantitative role of the various cell types (parenchymal, Kupffer and endothelial cells) to the receptor-mediated uptake by the liver in vivo, were until recently hampered by the applied cell isolation procedures during which bound and/or internalized substances were processed and lost from the cells. The procedure as outlined in scheme 1 prevents such a processing because both liver perfusion, cell separation and cell purification are performed at a low temperature (at 8° C). In addition one is able to assess the recovery by comparing the radioactivity in the purified parenchymal, endothelial and Kupffer cells (all obtained from one liver) to the radioactivity originally present in the whole rat liver. The method was evaluated using125 I-asialofetuin as the substrate (Fig. 1) and compared to quantitative autoradiographic data [1].
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Hubbard AL, Wilson G, Ashwell G, Stukenbrok H (1979) J Cell Biol 83: 47–64
Harkes L, Van Berkel TJC (1984) Biochem J 224: 21–27
Goldstein JL, Ho YK, Basu SK, Brown MS (1979) Proc Natl Acad Sci USA 76: 333–337
Henriksen T, Mahoney EM, Steinberg D (1981) Proc Natl Acad Sci USA 78: 6499–6503
Van Berkel TJC, Nagelkerke JF, Harkes L, Kruijt JK (1982) Biochem J 208: 493–503
Nagelkerke JF, Barto KP, Van Berkel TJC (1983) J Biol Chem 258: 12221–12227
Mommaas-Kienhuis AM, Nagelkerke JF, Vermeer BJ, Daems WT, Van Berkel TJC (1985) Eur J Cell Biol 38: 42–50
Kempen HJM, Spanjer HH, Van Berkel THJC (1985) Eur J Cell Biol in press
Van Berkel TJC (1982) in „Metabolic Compartmentation“ (H. Sies, ed) Academic Press Inc London pp 437–482
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1986 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
van Berkel, T.J.C., Kruijt, J.K., Nagelkerke, J.F., Harkes, L. (1986). Cellular Localization of Lipoprotein Receptors in Liver. In: Greten, H., Windler, E., Beisiegel, U. (eds) Receptor-Mediated Uptake in the Liver. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-70956-2_7
Download citation
DOI: https://doi.org/10.1007/978-3-642-70956-2_7
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-16181-3
Online ISBN: 978-3-642-70956-2
eBook Packages: Springer Book Archive