Intracellular Degradation of Asialoglycoproteins in Rat Hepatocytes Studied by Fractionation in Nycodenz Gradients

  • T. Berg
  • G. Kindberg
  • T. Ford
  • R. Blomhoff
Conference paper

Abstract

Information about the intracellular transport of endocytosed ligands may be obtained by subcellular fractionation studies. We have used this approach to follow the intracellular path of asialoglycoproteins in isolated rat liver parenchymal cells (PC). In these studies asialoorosomucoid labeled with125 I-tyramine cellobiose (125I-TC-AOM) was used. When ligands labeled in this way are degraded, the labeled degradation products are trapped in the lysosomes as they do not penetrate the lysosomal membrane. Therefore, in subcellular fractionation studies the labeled degradation products may serve as markers for the organeltes in which they are formed.

Keywords

Sucrose Albumin Fractionation Colchicine Collagenase 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1986

Authors and Affiliations

  • T. Berg
    • 1
  • G. Kindberg
    • 1
  • T. Ford
    • 1
  • R. Blomhoff
    • 1
  1. 1.Institute for Nutrition ResearchUniversity of OsloOslo 3Norway

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