Intracellular Degradation of Asialoglycoproteins in Rat Hepatocytes Studied by Fractionation in Nycodenz Gradients
Information about the intracellular transport of endocytosed ligands may be obtained by subcellular fractionation studies. We have used this approach to follow the intracellular path of asialoglycoproteins in isolated rat liver parenchymal cells (PC). In these studies asialoorosomucoid labeled with125 I-tyramine cellobiose (125I-TC-AOM) was used. When ligands labeled in this way are degraded, the labeled degradation products are trapped in the lysosomes as they do not penetrate the lysosomal membrane. Therefore, in subcellular fractionation studies the labeled degradation products may serve as markers for the organeltes in which they are formed.
KeywordsParenchymal Cell Endocytic Vesicle Extracellular Ligand Norwegian Council Lysosomal Marker Enzyme
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