Abstract
Imaging of malignancy in patients using radiolabelled antibodies has now been successfully demonstrated in various tumor types. These include colorectal cancer [2, 11, 14], breast cancer [7], malignant melanoma [13], and ovarian cancer [22]. However, although rodent sarcomas were among the first tumors to be localised using specific antitumor antibodies in animals [12, 18], there is little clinical data concerning immunoscintigraphy in bone and soft tissue tumors. In 1981 a monoclonal antibody raised against an osteosarcoma cell line, 791T, was described by Embleton et al. [4]. This monoclonal antibody showed no reaction with fibroblasts, human red blood cells or peripheral mononuclear cells. However, it reacted strongly against the immunizing osteosarcoma cell line and other osteosarcoma cell lines. It was demonstrated that with the addition of iodine as a radiolabel the antibody maintained its activity against osteosarcoma cell lines. In vivo studies using 791T tumors xenografted into immunodeprived mice showed that radiolabelled 791T/36 localised within the tumors, demonstrated both by external gamma scintigraphy and by direct measurement of uptake when a localisation index of between 4 and 5.6 was found compared with radiolabelled normal mouse immunoglobulin (NMI) [17]. This localisation prompted clinical studies using this radiolabelled antibody in patients with bone and soft tissue and a single case was reported showing clear localisation in a patient with osteosarcoma [8].
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References
Bacci G, Picci D, Caldreni P, Figus E, Borgh A (1982) Full lung tomograms and bone scanning in the initial work up of patients with osteosarcoma: a review of 126 patients. Eur J Cancer Clin Oncol 18:967–971
Chatal JF, Saccavini J-C, Fumoleau P, Douillard J-Y, Curtet C, Kremer M et al. (1984) Immunoscintigraphy of colon carcinoma. J Nucl Med 25(3):307–314
Eilber FR, Eckhardt J, Morton DL (1984) Advances in the treatment of sarcomas in the extremity. Cancer 54:2695–2701
Embleton MJ, Gunn B, Byers VS, Baldwin RW (1981) Antitumour reactions of monoclonal antibody against a human osteogenic sarcoma cell line. Br J Cancer 43:582–587
Embleton MJ, Rowland GF, Simmonds RG, Jacobs E, Marsden CH, Baldwin RW (1983) Selective cytotoxicity against human tumour cells by a vindesine-monoclonal antibody conjugate. Br J Cancer 47:43–49
Enneking WF, Kagan A (1975) ‘Skip’ metastases in osteosarcoma. Cancer 36(6): 2192–2205
Epenetos AA, Mather S, Granowska M, Nimmon CC, Hawkins LR, Britton K et al. (1982) Targetting of iodine-123-labelled tumor-associated monoclonal antibodies to ovarian, breast and gastrointestinal tumors. Lancet ii:999–1004
Farrands PA, Perkins AC, Sully L, Hopkins JS, Pimm MV, Baldwin RW, Hardcastle JD (1983) Localisation of human osteosarcoma by antitumour monoclonal antibody. J Bone Joint Surg 65-B:638–640
Fraker PJ, Speck JC (1978) Protein and cell membrane iodination with a sparingly soluble chloroamide 1,3,4,6-tetrachloro-3,6-diphenyl-glycouril. Biochem Biophys Res Commun 80:849–857
Garnett MC, Embleton MJ, Jacobs E, Baldwin RW (1983) Preparation and properties of a drug-carrier-antibody conjugate showing selective antibody-directed cytotoxicity in vitro. Int J Cancer 31:661–670
Goldenberg DM, DeLand F, Kim E, Bennett S, Primus FJ, Van Nagell JR, Estes N et al. (1978) Use of radiolabelled antibodies to carcinoembryonic antigen for the detection and localisation of diverse cancers by external photoscanning. N Engl J Med 298(25): 1384–1388
Korngold L, Pressman D (1954) The localisation of anti-lymphosarcoma in the Murphy lymphosarcoma of the rat. Cancer Res 14:96–99
Larson SM, Brown JP, Wright PW, Carrasquillo JA, Hellstrom I, Hellstrom KE (1983) Imaging of melanoma with I-131 labelled monoclonal antibodies. J Nucl Med 24(2): 123–129
Mach JP, Bucheggar F, Forni M, Ritschard J, Berche C, Lumbroso JD et al. (1981) Use of radiolabelled monoclonal anti-CEA antibodies for the detection of human carcinomas by external photoscanning and tomoscintigraphy. Immunol Today 2(12):239–249
Perkins AC, Whalley DR, Hardy JG (1984) Physical approach for the reduction of dual radionuclide image subtraction artefacts in immunoscintigraphy. Nucl Med Commun 5:501–512
Perkins AC, Pimm MV, Birch MK (1985) The preparation and characterisation of 111In- labelled monoclonal antibody for tumour immunoscintigraphy. Eur J Nucl Med 10:296–301
Pimm MV, Embleton MV, Perkins AC, Price MR, Robins RA, Robinson GR, Baldwin RW (1982) In vivo localisation of antiosteogenic sarcoma 791T monoclonal antibody in osteogenic sarcoma xenografts. Int J Cancer 30:75–85
Pressman D, Korngold L (1953) The in vivo localisation of anti-Wagner osteogenic sarcoma. Cancer 6:619–623
Roth JA, Restropo C, Scuderi P, Baldwin RW, Reichert CM, Hosoi S (1984) Analysis of antigenic expression by primary and autologous metastatic sarcoma using monoclonal antibodies. Cancer Res 44:5320–5325
Rowland GF, Axton CA, Baldwin RW, Brown JP, Corvalan JRF, Embleton MJ et al. (1985) Antitumor properties of vindesine monoclonal antibody conjugates. Cancer Immunol Immunother 19:1–17
Simm FR, Pritchard DJ (1980) Computer tomography for the detection of pulmonary metastases in patients with osteosarcoma. Proc Am Assoc Cancer Res 21:148
Symonds EM, Perkins AC, Pimm MV et al. (1985) Clinical implications for immunoscintigraphy in patients with ovarian malignancy. A preliminary study using monoclonal antibody 791T/36. Br J Obstet Gynaecol 92:270–276
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© 1986 Springer-Verlag Berlin Heidelberg
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Armitage, N.C., Perkins, A.C., Pimm, M.V., Baldwin, R.W., Hardcastle, J.D. (1986). Imaging of Bone and Soft Tissue Tumors Using an Antitumor Monoclonal Antibody. In: Winkler, C. (eds) Nuclear Medicine in Clinical Oncology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-70947-0_31
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DOI: https://doi.org/10.1007/978-3-642-70947-0_31
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-16164-6
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