Implications of Proteases and Protease Inhibitors in Neurite Outgrowth
There is increasing experimental evidence that the proteolytic activity associated with cells can strongly affect their behavior. The role of cell-surface protease activity in cell migration has been stressed in many developmental systems [1,2]. Migrating cells, including cells transformed by oncogenic viruses, have more cell-associated proteolytic activity than stationary, differentiated cells [3,4]. Cell-associated proteolytic activity is usually estimated by measuring cell-derived plasminogen activators . Plasminogen activators are serine proteases that convert plasminogen to plasmin, which then degrades substrates such as fibrin  or casein . In the nervous system, an increased amount of plasminogen activator can be attributed to cultured, cerebellar granule neurons if the dissociation and the initiation of the cultures take place at the developmental stage at which these cells are migrating . It was also demonstrated that, in neuroblastoma cells, plasminogen activator activity can be preferentially localized at the growth cone when neurite outgrowth is induced by treatment with dibutyryl cyclic AMP . These results have suggested that such proteolytic activity is required for the migratory behavior of the growth cone during neurite outgrowth .
KeywordsPlasminogen Activator Proteolytic Activity Neurite Outgrowth Growth Cone Cerebellar Granule Neuron
Unable to display preview. Download preview PDF.
- 14.Monard D, Murato K, Guenther J (1985) Glia-induced neurite extension as a model to detect some biochemical interactions between glia and neurons. In: Dumont JE, Hamprecht B, Nunez J (eds) Hormones and cell regulation, vol IX. Elsevier Science Publ, Amsterdam, pp 391–398Google Scholar
- 15.Guenther J, Nick H, Monard D (1985) A glia-derived neurite-promoting factor with protease inhibitory activity. EMBO 4:1963–1966Google Scholar
- 16.Lindner J, Guenther J, Nick H, Zinser G, Antonicek H, Schachner M, Monard D (1985) Modulation of granule cell migration by a glia-derived protein. In preparationGoogle Scholar
- 19.Murato K, Monard D (1985) In preparationGoogle Scholar
- 25.Patterson PH (1985) On the role of proteases, their inhibitors and the extracellular matrix in stimulating neurite outgrowth. J Neurochem 44: Suppl s9BGoogle Scholar
- 26.Patterson PH (1985) J Physiol (Paris) in pressGoogle Scholar