Laser-Flash Photoaffinity Labeling of Acetylcholine Receptors with Millisecond Time Resolution
The function of membrane-bound receptors is the transduction of signals from the outside of a cell to the inside. The signal itself and therefore the transduction process, too, are transient molecular events. In the case of the nicotinic acetylcholine receptor (nAChR) the signal consists of acetylcholine molecules binding very rapidly (diffusion controlled, Maelicke 19 84) to the receptor protein. The transduction process consists of a change of the electrical properties of the receptor membrane. It is based on ion currents through transiently opened ion channels formed by the receptor protein (Hille 1984).
KeywordsQuartz Rubber Electrophoresis Syringe Polypeptide
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