Laser-Flash Photoaffinity Labeling of Acetylcholine Receptors with Millisecond Time Resolution

  • A. Fahr
  • S. Hellmann
  • L. Lauffer
  • P. Muhn
  • F. Hucho
Conference paper
Part of the Colloquium der Gesellschaft für Biologische Chemie 18.–20. April 1985 in Mosbach/Baden book series (MOSBACH, volume 36)


The function of membrane-bound receptors is the transduction of signals from the outside of a cell to the inside. The signal itself and therefore the transduction process, too, are transient molecular events. In the case of the nicotinic acetylcholine receptor (nAChR) the signal consists of acetylcholine molecules binding very rapidly (diffusion controlled, Maelicke 19 84) to the receptor protein. The transduction process consists of a change of the electrical properties of the receptor membrane. It is based on ion currents through transiently opened ion channels formed by the receptor protein (Hille 1984).


Acetylcholine Receptor Nicotinic Acetylcholine Receptor Competitive Antagonist Voltage Sensitivity Photoaffinity Label 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1985

Authors and Affiliations

  • A. Fahr
    • 1
  • S. Hellmann
    • 2
  • L. Lauffer
    • 3
  • P. Muhn
    • 4
  • F. Hucho
    • 4
  1. 1.Institut für Atom- und Festkörperphysik, Abtlg. BiophysikFreie Universität BerlinBerlin 33Deutschland
  2. 2.Max-Planck-Institut für ErnährungsphysiologieDortmundGermany
  3. 3.Medical School, Dept. of Biochemistry and BiophysicsUCSFSan FranciscoUSA
  4. 4.Institut für Biochemie, Arbeitsgruppe NeurochemieFreie Universität BerlinBerlin 33Deutschland

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