Advertisement

Rapid assessment of stress effects on plant leaves by chlorophyll fluorescence measurements

  • U. Schreiber
  • W. Bilger
Part of the NATO ASI Series book series (volume 15)

Abstract

Chlorophyll fluorescence serves as an intrinsic indicator of the photosynthetic reactions in the chloroplasts of green plants. The relationship between chlorophyll fluorescence and the mechanisms of photosynthesis have been the subject of a great number of investigations, since Kautsky discovered (Kautsky and Hirsch 1931) that fluorescence intensity in green leaves displays characteristic changes upon illumination (Kautsky effect - for reviews, see Papageorgiou 1975; Lavorel and Etienne 1977; Krause and Weis 1984). For almost half a century fluorescence has been mainly a tool for biophysically oriented researchers in studies of the primary processes of photosynthesis. Practical applications of the Kautsky effect in ecophysiological work had been limited by the availability of suitable instrumentation and by the complexity of the fluorescence information obtained in vivo. In recent years considerable efforts have been put into the development of field oriented fluorescence equipment and into the development of the methodology for analysing fluorescence data from intact leaves.

Keywords

Chlorophyll Fluorescence Relative Water Content Saturation Pulse Intact Leaf Variable Fluorescence 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Barber J (1983) Membrane conformational changes due to phosphorylation and the control of energy transfer in photosynthesis. Photobiochem Photobiophys 5: 181–190Google Scholar
  2. Bennett J, Steinback KE, Arntzen CJ (1980) Chloroplast phosphoproteins: Regulation of excitation energy transfer by phosphorylation of thylakoid membrane polypeptides. Proc Natl Acad Sci 77: 5253–5257PubMedCrossRefGoogle Scholar
  3. Beyschlag W (1984) Photosynthese und Wasserhaushalt von Arbutus unedo L. im Jahreslauf am Freilandstandort in Portugal. Gaswechselmessungen unter natürlichen Bedingungen und experimentelle Faktorenanalyse. Dissertation, WürzburgGoogle Scholar
  4. Bilger W, Schreiber U (1987) Energy dependent quenching of dark-level chlorophyll fluorescence in intact leaves. In: Govindjee et al. (eds), Excitation Energy and Electron Transfer in Photosynthesis, Photosynthesis Research Supp. Vol. Martinus Nijhoff/Dr. W. Junk Publishers, DordrechtGoogle Scholar
  5. Bilger W, Schreiber U, Lange OL (1984) Determination of leaf heat resistance: Comparative investigation of chlorophyll fluorescence changes and tissue necrosis methods. Oecologia 63: 256–262CrossRefGoogle Scholar
  6. Björkman O (1981) Responses to different quantum flux densities. In: Lange OL, Nobel PS, Osmond CB, Ziegler H (eds) Encyclopedia of Plant Physiology, NS vol 12A. Springer, Berlin-Heidelberg-New York, pp 57–107Google Scholar
  7. Björkman O, Powles SB (1984) Inhibition of photosynthetic reactions under water stress: Interaction with light level. Planta 161: 490–504CrossRefGoogle Scholar
  8. Bradbury M, Baker NR (1981) Analysis of the slow phases of the in vivo chlorophyll fluorescence induction curve. Changes in redox state of photosystem II electron acceptors and fluorescence emission from photosystems I and II. Biochim Biophys Acta 635: 542–551PubMedCrossRefGoogle Scholar
  9. Critchley C, Smillie RM (1981) Leaf chlorophyll fluorescence as an indicator of photoinhibition in Cucumis sativus L. Aust J Plant Physiol 8: 133–141CrossRefGoogle Scholar
  10. Dietz KJ, Schreiber U, Heber U (1985) The relationship between the redox state of QA and photosynthesis in leaves at various carbon dioxide, oxygen and light regimes. Planta 166: 219–226CrossRefGoogle Scholar
  11. Duysens LNM, Sweers HE (1963) Mechanism of two photochemical reactions in algae as studied by means of fluorescence. In: Studies on Microalgae and Photosynthetic Bacteria. University of Tokyo Press, Tokyo, pp 353–372Google Scholar
  12. Govindjee, Downton WJS, Fork DC, Armond PA (1981) Chlorophyll a fluorescence transient as an indicator of water potential of leaves. Plant Sci Lett 20: 191–194CrossRefGoogle Scholar
  13. Havaux M, Lannoye R (1983) Chlorophyll fluorescence induction: A sensitive indicator of water stress in maize plants. Irrig Sci 4: 147–151Google Scholar
  14. Kautsky H, Franck U (1943) Chlorophyllfluoreszenz und Kohlensäureassimilation XI. Die Chlorophyllfluoreszenz von Ulva lactuca und ihre Abhängigkeit von Narkotika, Sauerstoff und Kohlendioxyd. Biochem Z 315: 176–206Google Scholar
  15. Kautsky H, Hirsch A (1931) Neue Versuche zur Kohlenstoffassimilation. Naturwissenschaften 19: 964CrossRefGoogle Scholar
  16. Kautsky H, Hirsch A (1934) Das Fluoreszenzverhalten grüner Pflanzen. Biochem Z 274: 422–434Google Scholar
  17. Krause GH, Briantais JM, Vernotte C (1982) Photoinduced quenching of Chlorophyll fluorescence in intact chloroplasts and algae. Resolution into two components. Biochim Biophys Acta 679: 116–124CrossRefGoogle Scholar
  18. Krause GH, Köster S, Wong SC (1985) Photoinhibition of photosynthesis under anaerobic conditions studied with leaves and chloroplasts of Spinacia oleracea L. Planta 165: 430–438CrossRefGoogle Scholar
  19. Krause GH, Weis E (1984) Chlorophyll fluorescence as a tool in plant physiology. II. Interpretation of fluorescence signals. Photosynth Res 5: 139–157CrossRefGoogle Scholar
  20. Kyle DJ, Ohad I, Arntzen CJ (1984) Membrane protein damage and repair: Selective loss of a quinone-protein function in chloroplast membranes. Proc Natl Acad Sci USA 81: 4070–4074PubMedCrossRefGoogle Scholar
  21. Lavorel J, Etienne AL (1977) In vivo chlorophyll fluorescence. In: Barber J (ed) Primary Processes of Photosynthesis. Elsevier, Amsterdam, pp 203–268Google Scholar
  22. Monson RK, Williams GJ (1982) A correlation between photosynthetic temperature adaptation and seasonal phenology patterns in the shortgrass prairie. Oecologia 54: 58–62CrossRefGoogle Scholar
  23. Ögren E, Baker NR (1985) Evaluation of a technique for the measurement of chlorophyll fluorescence from leaves exposed to continuous white light. Plant, Cell & Environment 8: 539–547CrossRefGoogle Scholar
  24. Papageorgiou G (1975) Chlorophyll fluorescence: An intrinsic probe of photosynthesis. In: Govindjee (ed) Bioenergetics of Photosynthesis. Academic Press, New York, pp 319–371Google Scholar
  25. Powles SB (1984) Photoinhibition of photosynthesis induced by visible light. Ann Rev Plant Physiol 35: 15–44CrossRefGoogle Scholar
  26. Powles SB, Björkman O (1982) Photoinhibition of photosynthesis: Effect on chlorophyll fluorescence at 77K in intact leaves and in chloroplast membranes of Nerium oleander. Planta 156: 97–107CrossRefGoogle Scholar
  27. Quick WP, Horton P (1984) Studies on the induction of chlorophyll fluorescence quenching by redox state and transthylakoid pH gradient. Proc R Soc Lond B 217: 405–416Google Scholar
  28. Renger G, Schreiber U (1986) Practical applications of fluorometric methods to algae and higher plant research. In: Govindjee, Amesz J and Fork DC (eds) Light Emission by Plants and Bacteria. Academic Press, New York, in pressGoogle Scholar
  29. Samuelsson G, Lönneborg A, Rosenquist E, Gustafsson P, Öquist G (1985) Photoinhibition and reactivation of photosynthesis in the cyanobacterium Anacystis nidulans. Plant Physiol 79: 992–995PubMedCrossRefGoogle Scholar
  30. Schreiber U (1983) Chlorophyll fluorescence yield changes as a tool in plant physiology. I. The measuring system. Photosynth Res 4: 361–373Google Scholar
  31. Schreiber U (1986) Detection of rapid induction kinetics with a new type of high frequency modulated chlorophyll fluorometer. Photosynth Res 9: 261–272CrossRefGoogle Scholar
  32. Schreiber U, Berry JA (1977) Heat-induced changes of chlorophyll fluorescence in intact leaves correlated with damage of the photosynthetic apparatus. Planta 136: 233–238CrossRefGoogle Scholar
  33. Schreiber U, Schliwa U, Bilger W (1986) Continuous recording of photochemical and non-photochemical fluorescence quenching with a new type of modulation fluorometer. Photosynth Res 9Google Scholar
  34. Sharkey TD (1985) Photosynthesis in intact leaves of C3 plants: Physics, physiology and rate limitations. Bot Rev 51: 53–105CrossRefGoogle Scholar
  35. Smillie RM, Nott R (1979) Heat injury in leaves of alpine, temperate and tropical plants. Aust J Plant Physiol 6: 135–141CrossRefGoogle Scholar
  36. Tenhunen JD, Weber J, Yocum C, Gates DM (1976) Development of a photosynthesis model with an emphasis on ecological applications. II. Analysis of a data set describing the PM surface. Oecologia (Berl) 26: 101–119CrossRefGoogle Scholar
  37. Velthuys BR, Amesz J (1974) Charge accumulation at the reducing side of system II of photosynthesis. Biochim Biophys Acta 333: 85–94PubMedCrossRefGoogle Scholar
  38. Weis E (1984) Short term acclimation of spinach to high temperatures. Effect on chlorophyll fluorescence at 293 and 77K in intact leaves. Plant Physiol 74: 402–407PubMedCrossRefGoogle Scholar
  39. Weis E (1985) Light- and temperature-induced changes in the distribution of excitation energy between photosystem I and photosystem II in spinach leaves. Biochim Biophys Acta 807: 118–126CrossRefGoogle Scholar
  40. Wiltens J, Schreiber U, Vidaver W (1978) Chlorophyll fluorescence induction: An indicator of photosynthetic activity in marine algae undergoing desiccation. Can J Bot 56: 2787–2794CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1987

Authors and Affiliations

  • U. Schreiber
    • 1
  • W. Bilger
    • 1
  1. 1.Botanisches Institutder Universität WürzburgWürzburgFederal Republic of Germany

Personalised recommendations