The Action of Spider Toxins on the Insect Nerve-Muscle System

  • P. N. R. Usherwood
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)

Abstract

In the search for new classes of pesticides it is perhaps surprising that only relatively scant attention has been given to the natural products found in the venoms of a variety of insect predators. Extensive use has been made of vertebrate and invertebrate toxins to gain information on the structure and function of central and peripheral nervous systems across the animal kingdom but for a variety of reasons these are of little interest to the chemical industry. However, many insect predators produce venoms, most of which remain largely uncharacterized, which may well contain active principles of commercial interest. Insect neurobiologists have compelling reasons to ponder over the possible potential of these compounds as research tools since many of the developments which recently have taken place in neuroscience have depended upon the use of venoms and toxins. For example, toxins from snake and spider venoms have been used to study transmitter storage, release and turnover at peripheral and central synapses and the snake toxin, α-bungarotoxin has been particularly instrumental in providing an understanding of the molecular properties of the nicotinic acetylcholine receptor protein.

Keywords

HPLC Depression Polypeptide Resis Acetylcholine 

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References

  1. Barnard EA (1982) Isolation of receptors from the central nervous system. In: Neuropharmacology of insects. Ciba Found Symp 88. Pitman, LondonGoogle Scholar
  2. Bateman A, Boden P, Dell A, Duce IR, Quicke DLJ, Usherwood PNR (1985) Postsynaptic block of a glutamatergic synapse by low molecular weight fractions of spider venom. Brain Res 339, 237–244PubMedCrossRefGoogle Scholar
  3. Boden P, Duce IR, Usherwood PNR (1984) Activation-induced postsynaptic block of insect nerve- muscle transmission by the low molecular weight fraction of spider venom. Br J Pharmacol, 82, 221 pGoogle Scholar
  4. Clark RB, Gration KAF, Usherwood PNR (1979) Desensitization of glutamate receptors on innervated and denervated muscle fibres. J Physiol (London) 290: 551–568Google Scholar
  5. Clark RB, Donaldson PL, Gration KAF, Lambert JJ, Piek T, Ramsey RL, Spanjer W, Usherwood PNR (1982) Block of locust muscle glutamate receptors by δ-philanthotoxin occurs after receptor activation. Brain Res 241: 105–114PubMedCrossRefGoogle Scholar
  6. Clements AN, May TE (1974) Studies on locust neuromuscular physiology in relation to glutamic acid. J Exp Biol 60: 673–705PubMedGoogle Scholar
  7. Cull-Candy SG, Neal H, Usherwood PNR (1973) Action of black widow spider venom on an aminergic synapse. Nature (London) 241: 353–354CrossRefGoogle Scholar
  8. Hoyle G (1955) Neuromuscular mechanisms of a locust skeletal muscle. Proc R Soc London Ser B 143: 346 - 367Google Scholar
  9. Kawai N, Niwa A, Abe T (1982a) Spider venom contains specific receptor blocker of glutaminergic synapses. Brain Res 247:169–171Google Scholar
  10. Kawai N, Niwa A, Abe T (1982b) Effects of spider toxin on glutaminergic synapses in the mammalian brain. Biomed Res 3:353–355Google Scholar
  11. Kawai N, Niwa A, Abe T (1983) Specific antagonism of the glutamate receptor by an extract from the spider Araneus ventricosus. Toxicon 21: 438–440PubMedCrossRefGoogle Scholar
  12. Lee CY (1970) Elapid neurotoxins and their mode of action. Clin Toxicol 3: 457–472PubMedCrossRefGoogle Scholar
  13. Mathers DA, Usherwood PNR (1976) Concanavalin A blocks desensitization of glutamate receptors on insect muscle fibres. Nature (London) 259: 409–411CrossRefGoogle Scholar
  14. Mathers DA, Usherwood PNR (1978) Effects of concanavalin A on junctional and extrajunctional L-glutamate receptors on locust skeletal muscle fibres. Comp Biochem Physiol 59 C:151–155Google Scholar
  15. Piek T (1966a) Site of action of venom of Microbracon hebetor Say (Braconidae, Hymenoptera). J Insect Physiol 12:561–568Google Scholar
  16. Piek T (1966b) Site of action of the venom of the digger wasp Philanthus triangulum F on the fast neuromuscular system of the locust. Toxicon 3:191–198Google Scholar
  17. Piek T (1969) Action of the venom of Microbracon hebetor Say on the hyperpolarizing potentials in a skeletal muscle of Philosamia cynthia Hubn. Comp Gen Pharmac 1: 117–120Google Scholar
  18. Piek T (1984) Insect venoms and toxins. In: Kerkut GA, Gilbert LI (eds) Comprehensive insect physiology, biochemistry and pharmacology, vol XI. Pharmacology. Pergamon Press, Oxford New YorkGoogle Scholar
  19. Piek T, Spanjer W (1978) Effects and chemical characterization of some paralyzing venoms of solitary wasps. In: Shankland DL, Hollingworth RM, Smyth T (eds) Pesticide and venom neurotoxicity. Plenum Press, New York LondonGoogle Scholar
  20. Piek T, Mantel P, Engels E (1971) Neuromuscular block in insects caused by the venom of the digger wasp Philanthus triangulum F. Comp Gen Pharmacol 2: 317–331PubMedCrossRefGoogle Scholar
  21. Tashmukhamedov BA, Usmanov PB, Kazakov I, Kalikulov D, Yukelson LY, Atakuziev BU (1983) Effects of different spider venoms on artificial and biological membranes. In: Toxins as tools in neurochemistry. de Gruyther, Berlin New York, pp. 312–323Google Scholar
  22. Usherwood PNR, Machili P (1966) Chemical transmission at the insect excitatory neuromuscular synapse. Nature (London) 210: 634–636CrossRefGoogle Scholar
  23. Usherwood PNR, Machiii P (1968) Pharmacological properties of excitatory neuromuscular synapses in the locust. J Exp Biol 49: 341–361Google Scholar
  24. Usherwood PNR, Duce IR, Boden P (1984) Slowly-reversible block of glutamate receptor-channels by venoms of the spiders, Argiope trifasciata and Araneus gemma. J Physiol (Paris) 79: 241–245Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1985 1985

Authors and Affiliations

  • P. N. R. Usherwood
    • 1
  1. 1.Department of ZoologyNottingham UniversityNottinghamUK

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