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Interaction of the Rous Sarcoma Virus Protein pp60src with the Cellular Proteins pp50 and pp90

  • J. S. Brugge
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 123)

Abstract

Oncogenic retroviruses cause multiple and profound alterations in the morphology, metabolism, and growth control of cells. In most retrovirus infected cells, all of these complex changes in the cellular phenotype are mediated by a single virus-encoded gene product, referred to as the transforming protein. Rous sarcoma virus (RSV) has proven to be an ideal system for the analysis of events which occur following oncogenic transformation by retroviruses. RSV induces rapid sarcoma production after injection of virus in vivo and efficient and rapid transformation of cells in culture (HANAFUSA 1977). The transforming gene of RSV encodes a protein of Mr 60 000 which has been designated pp60 src (Brugge and Erikson 1977; Purchio et al. 1978). Genetic studies of viruses encoding mutant src gene products which induce a partially transformed phenotype suggest that interactions between pp60 src and multiple cellular targets are required to elicit a fully transformed phenotype (review, Sefton and Hunter 1984). This review will discuss one such interaction between pp60 src and host cell proteins. This interaction occurs between newly synthesized molecules of pp60 src and two cellular proteins of Mr 90 000 (pp90) and 50 000 (pp50). The kinetics and localization of this interaction suggest that the cellular pp90 and pp50 proteins are involved in some aspect of the processing of pp60 src before it reaches its residence in the plasma membrane (Courtneidge and Bishop 1982; BRUGGE et al. 1983). pp90 and pp50 have also been shown to associate with many retrovirus-encoded transforming proteins other than pp60 src (Lipsich et al. 1982; Adkins et al. 1982). This suggests that pp50 and pp90 may play a common role in the events which take place after transformation by at least one class of retrovirus transforming proteins.

Keywords

Uninfected Cell Rous Sarcoma Virus Chicken Embryo Fibroblast Nonpermissive Temperature Glycerol Gradient 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1986

Authors and Affiliations

  • J. S. Brugge
    • 1
  1. 1.Department of MicrobiologyState University of New York at Stony BrookStony BrookUSA

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