Transfer and Random Integration of Tn5 in Azospirillum

  • M. Vanstockem
  • K. Michiels
  • J. Vanderleyden
  • A. Van Gool
Conference paper


A method is described for the isolation of at random Tn5 insertion mutants of Azospirillum spp. For this purpose a set of plasmid vectors of Tn5, that are suicidal in Rhizobium and Agrobacterium species, were introduced in A. brasilense Sp7 and A. lipoferum SpBrl7 by conjugation. The plasmids pSUP2021 and pGS9 are suicidal in these Azospirillum strains as shown by hybridization experiments of digested total DNA of twenty bona fide Kmr ex-conjugants with 32P labeled ColEl:: Tn5. Kanamycm-resistant colonies were detected at a frequency of 10−8 per recipient cell.


Azospirillum transposon mutagenesis suicide plasmids Southern hybridization 


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Copyright information

© Springer-Verlag Berlin Heidelberg 1985

Authors and Affiliations

  • M. Vanstockem
    • 1
  • K. Michiels
    • 1
  • J. Vanderleyden
    • 1
  • A. Van Gool
    • 1
  1. 1.F.A. Janssens Laboratory of GeneticsCatholic University of LeuvenHeverleeBelgium

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