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Problems and Prospects of Site-Directed Transposon Mutagenesis in Azospirillum

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Azospirillum III

Summary

Different vectors and transposons were tested to obtain site-directed nif- mutants of A. brasilense. Tn5 proved more suitable than Tn1725 in this respect. A novel method of site- directed nif-gene deletion formation in combination with resistance-gene-cartridge mutagenesis is also reported. Keywords: nif-genes, transposon mutagenesis, cloning, cartridge mutagenesis, nif- mutants.

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References

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Author information

Authors and Affiliations

  1. Lehrstuhl für Genetik, Universität Bayreuth, Universitätsstraße 30, D-8580, Bayreuth, Germany

    Mahavir Singh & Walter Klingmüller

Authors
  1. Mahavir Singh
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  2. Walter Klingmüller
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Editor information

Editors and Affiliations

  1. Lehrstuhl für Genetik, Universität Bayreuth, Universitätsstraße 30, 8580, Bayreuth, Germany

    Walter Klingmüller

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© 1985 Springer-Verlag Berlin Heidelberg

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Singh, M., Klingmüller, W. (1985). Problems and Prospects of Site-Directed Transposon Mutagenesis in Azospirillum. In: Klingmüller, W. (eds) Azospirillum III. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-70791-9_3

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  • DOI: https://doi.org/10.1007/978-3-642-70791-9_3

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-70793-3

  • Online ISBN: 978-3-642-70791-9

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