Summary
Previous experiments from this laboratory showed that Azospirillum brasilense Sp 7 can grow anaerobically with either nitrate, nitrite or nitrous oxide as respiratory electron acceptors. Molar growth yield measurements now indicate that Azospirillum obtains the same amount of energy with NO −2 and O2 as respiratory electron acceptors but only 1/3 of that with NO −3 . Dissimilatory nitrite reductase catalyzing the reduction of NO −2 to N2O is shown to be a cytochrome c, d containing enzyme. A soluble dissimilatory nitrite reductase preparation also catalyzes the formation of nitric oxide which is probably an artifact of the cell-free system. The expression of dissimilatory N2O-reductase in Azospirillum is dependent on Cu in the medium.
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References
Okon,Y. (1984). In Veeger, C. and Newton, W.E. (eds.): Advances in Nitrogen Fixation, pp. 303–309, Nijhoff-Junk-Pudoc (Publishers), The Hague, Boston, Lancaster.
Döbereiner, J. (1983). In Klingmüller,W.(ed.) Azospirillum II, Genetics, Physiology,Ecology, EXS 48,pp. 9–23,Birkhäuser,Basel
Van Berkum,P. and Kayser,H.H. (1985). Appl. Environ.Microbiol. 49, 772–777.
Nelson,L.M. and Knowles,R. (1978). Can. J. Microbiol. 24, 1395–1403.
Tibelius, K.H. and Knowles, R. (1984). J. Bact. 157, 84–88.
Zimmer, W.,Penteado Stephan,M. and Bothe, H. (1984)-Arch. Microbiol. 138, 206–211.
Penteado Stephan,M., Zimmer, W and Bothe, H. (1984). Arch. Microbiol. 138, 212–216.
Neuer, G., Kronenberg, A. and Bothe, H. (1985). Arch. Microbiol. 141, 364–370.
Tarrand J.J., Krieg, N. R., Döbereiner, J. (1978). Can. J. Microbiol. 24, 967–980.
Zimmer W., Danneberg, G. and Bothe, H. accepted by Current Microbiol.
Thauer, R.K., Jungermann, K. and Decker,K. (1977). Bacteriol. Reviews 41, 100–180.
Bryan, B.A. (1981). In Delwiche, C. C.(ed.); Denitrification, nitrification and atmospheric nitrous oxide, pp. 67–84, Wiley, New York, Chicester, Brisbane, Toronto.
Shapleigh, J. P. and Payne, W. J. (1985). FEMS Microbiol. Letters 26, 275–279.
Lam, Y. and Nicholas, D.J.D. (1969). Biochim. Biophys. Acta 180, 459–472.
Newton, N. (1969). Biochim. Biophys. Acta 185, 316–331.
Zumft, W.G. and Vega, J. M. (1969). Biochim. Biophys. Acta 548, 484–499.
Kim, C.-H. and Hollocher, T.C. (1983). J. Biol. Chem. 258, 4861–4863.
Zumft, W.G. and Matsubara, T. (1982). FEBS Letters 148, 107–112.
Zumft, W.G. Coyle, C. L., and Frunzke, K. (1985). FEBS Letters 183, 240–244.
Snyder, S.W. and Hollocher T.C. (1984). Biochem. Biophys. Res. Comm. 119, 588–592.
Long, C. (ed.). (1961). Biochemists’ Handbook, p. 96, Van Nostrand, Princeton, Toronto, New York, London.
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Danneberg, G., Zimmer, W., Bothe, H. (1985). Some Physiological and Biochemical Properties of Denitrification by Azospirillum Brasilense. In: Klingmüller, W. (eds) Azospirillum III. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-70791-9_13
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DOI: https://doi.org/10.1007/978-3-642-70791-9_13
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