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Construction of a Gene Bank of Azospirillum Brasilense

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Azospirillum III

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Summary

A gene bank of DNA from A.brasilense, strain Sp6, has been constructed in the single EcoRI and BgIII sites of plasmid pRK290. The vector was able to be transferred by conjugation from E.coli to A.brasilense by using the helper plasmid pRK2013 at a frequency of about 10−3per recipient cell. Clones carrying his and pyr genes have been identified by genetic complementation; the expression of A.brasilense in E.coli has been demonstrated. Keywords: gene bank, cloning, conjugation.

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Author information

Authors and Affiliations

  1. Dipartimento di Biologia Animale e Genetica, University of Florence, Via Romana 17, Florence, Italy

    R. Fani, M. Bazzicalupo, E. Gallori, L. Turbanti & M. Polsinelli

Authors
  1. R. Fani
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  2. M. Bazzicalupo
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  3. E. Gallori
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  4. L. Turbanti
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  5. M. Polsinelli
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Editor information

Editors and Affiliations

  1. Lehrstuhl für Genetik, Universität Bayreuth, Universitätsstraße 30, 8580, Bayreuth, Germany

    Walter Klingmüller

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© 1985 Springer-Verlag Berlin Heidelberg

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Fani, R., Bazzicalupo, M., Gallori, E., Turbanti, L., Polsinelli, M. (1985). Construction of a Gene Bank of Azospirillum Brasilense. In: Klingmüller, W. (eds) Azospirillum III. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-70791-9_1

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  • DOI: https://doi.org/10.1007/978-3-642-70791-9_1

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-70793-3

  • Online ISBN: 978-3-642-70791-9

  • eBook Packages: Springer Book Archive

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