Weight and Morphology of Bacteria Exposed to Antibiotics

  • V. Lorian
  • W. Tosch
  • D. Joyce
Conference paper


Exposure of bacteria to sub-MIC of various beta-lactam and other antibiotics results in morphologic alterations including a substantial increase in size. Staphylococci change into large cells with a diameter of 2–3 µm [1] and gram-negative bacilli grow into long filaments [2,3,4].


Agar Penicillin Bacillus Pseudomonas Ampicillin 


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  1. 1.
    Lorian V (1975) Some effects of subinhibitory concentrations of penicillin on the structure and division of staphylococci. Antimicrob Agents Chemother 7: 864–870PubMedGoogle Scholar
  2. 2.
    Gardner AD (1940) Morphological effects of penicillin on bacteria. Nature 146: 837–838CrossRefGoogle Scholar
  3. 3.
    Zimmerman SB, Stapley O (1976) Relative morphological effects induced by cefoxitin and other beta-lactam antibiotics in vitro. Antimicrob Agents Chemother 9: 318PubMedGoogle Scholar
  4. 4.
    Lorian V, Sabath LD (1972) Penicillins and cephalosporins; differences in morphologic effects on Proteus mirabilis. J Infec Dis 125: 560–564CrossRefGoogle Scholar
  5. 5.
    Lorian V (1980) Effects of subminimum inhibitory concentrations of antibiotics on bacteria. In: Lorian V (ed.) Antibiotics in laboratory medicine. Williams and Wilkins, Maryland, p 342–408Google Scholar
  6. 6.
    Robinow CF (1944) Cytological observations on Bact. coli, Proteus vulgaris and various aerobic spore-forming bacteria with special reference to the nuclear structures. J Hygiene 43: 413–423CrossRefGoogle Scholar
  7. 7.
    Everson-Pearse AG (1972) Histochemistry theoretical and applied. Williams and Wilkins, Maryland, p 1186–1192Google Scholar
  8. 8.
    Bahr CF, Engler WF, Mazzone HM (1976) Determination of the mass of viruses by quantitative electron microscopy. Quarterly Revs Biophys 9: 459–489CrossRefGoogle Scholar
  9. 9.
    Scullard G, Meynell E (1966) Bacterial mass measured with the MRC greywedge photometer. J Pathol Bacteriol 91: 608–612PubMedCrossRefGoogle Scholar
  10. 10.
    Lorian V, Atkinson B (1977) Comparison of the effects of mecillinam and 6- aminopenicillanic acid on Proteus mirabilis, Escherichia coli, and Staphylococcus aureus. Antimicrob Agents Chemother 11: 541–552PubMedGoogle Scholar
  11. 11.
    Amaral L, Viano I, Atkinson B, Lorian V (1981) Development, replication, and reversion of mecillinam-induced ovoid form of Salmonella typhimurium. Curr Microbiol 5: 207–211CrossRefGoogle Scholar
  12. 12.
    Lorian V, Atkinson B (1984) Bactericidal effect of polymorphonuclear leukocytes on antibiotic induced filaments of gram-negative bacilli. J Infect Dis 149: 719–727PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1985

Authors and Affiliations

  • V. Lorian
    • 1
  • W. Tosch
    • 2
  • D. Joyce
    • 1
  1. 1.Section of Microbiology and Epidemiology, Department of PathologyThe Bronx Lebanon Hospital CenterBronxUSA
  2. 2.Ciba GeigyBaselSwitzerland

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