Use of Lectins to Characterize Surface Alterations of β-lactam Resistant Mutants of Pseudomonas aeruginosa

  • G. Stübner
  • R. Marre
Conference paper


Lectins are defined as sugar binding proteins of plant origin with no detectable enzymatic activity [5]. They react with cellular bound or soluble carbohydrates selectively likened to the antigen antibody reaction [10,11]. They possess specifities for a wide range of carbohydrate structures [2,4,8,11]. Bacteria have a lot of surface components which are capable of interacting with lectins, e.g. teichoic acid, lipopolysaccharides, peptidoglycan, group specific antigens and hydrophobic areas of proteins of the outer membrane [2,3,11].


Neisseria Gonorrhoeae Carbohydrate Structure Teichoic Acid Antigen Antibody Reaction Agglutination Reaction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Connelly MC, Stein DC, Young FE, Morse SE, Allan PZ (1981) Interaction with lectins and differential wheat germ agglutinin binding of pyocin 103 sensitive—and resistant Neisseria gonorrhoeae. J Bacteriol 48: 796–803Google Scholar
  2. 2.
    Doyle R, Keller K (1984) Lectins in diagnostic microbiology. Eur J Clin Microbiol 3: 4–9PubMedCrossRefGoogle Scholar
  3. 3.
    Firon N, Ofek I, Sharon N (1983) Carbohydrate specifity of the surface lectins of Escherichia coli, Klebsiella pneumoniae, and Salmonella typhimurium. Carbohydrate Research 120: 235–250PubMedCrossRefGoogle Scholar
  4. 4.
    Goldstein IJ, Hayes CE (1978) The lectins: carbohydrate-binding proteins of plants and animals. In: Tipson RS, Horton D (ed) Advances in Carbohydrate Chemistry and Biochemistry, Academic Press, New York-San Francisco-London, p 127Google Scholar
  5. 5.
    Hart DA (1980) Lectins in biological systems: applications to microbiology. Am J Clin Nutr 33: 2416–2421PubMedGoogle Scholar
  6. 6.
    Matthew M, Harris AM, Marshall MJ, Ross GW (1975) The use of analytical isoelectric focusing for detection and identification of ß-lactamases. J Gen Microbiol 88: 169–178Google Scholar
  7. 7.
    Scatchard G (1949) The attraction of proteins for small molecules and ions. Ann NY Acad Sci 51: 660–672CrossRefGoogle Scholar
  8. 8.
    Sharon N, Lis H (1972) Lectins: cell agglutinating and sugar—specific proteins. Science 177: 949–959PubMedCrossRefGoogle Scholar
  9. 9.
    Stübner G (1984) Significance of lectins and lectin-receptors of bacteria and PMN for phagocytosis and intracellular killing. 18th Congress of the International Society of Blood Transfusion, Munich, July 22–27, 1984, Abstract S 37—0 3Google Scholar
  10. 10.
    Uhlenbruck G, van Mil A, Dreesbach K, Kich O (1982) Makrophagen und Phagocytose—Funtionsteste. Immun Infekt 10: 122–129PubMedGoogle Scholar
  11. 11.
    Uhlenbruck G, Groß R, Koch OM, Chun-Kyung L (1983) Die Bedeutung von Lektinen für den Adhäsionsmechanismus von Bakterien. Deutsches Ärzteblatt 80: 27–32Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1985

Authors and Affiliations

  • G. Stübner
    • 1
  • R. Marre
    • 2
  1. 1.Institut für Bakteriologie und HygieneKrankenhaus NordstadtHannoverGermany
  2. 2.Institut für Medizinische MikrobiologieMed. Hochschule LübeckLübeckGermany

Personalised recommendations