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Subinhibitory concentrations of antibiotics modulate amount of Shiga-like toxin produced by Escherichia coli

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The Influence of Antibiotics on the Host-Parasite Relationship II

Abstract

Many strains of Escherichia coli that cause enteric disease in a different way from the classical mechanism of enteroinvasiveness or elaboration of heat-labile or heat-stable enterotoxins produce a cytotoxin with biochemical and biological properties similar to the toxin of Shigella dysenteriae 1 (Shiga’s bacillus) [11]. This toxin is enterotoxic for rabbit intestinal segments, lethal for mice and rabbits, and cytotoxic for tissue culture cells [10]. Its potential medical relevance in humans is suggested by the strong correlation between toxin production and the ability of E. coli to cause bloody diarrhea, hemorrhagic colitis or hemolytic-uremic syndrome [9].

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References

  1. Achtman M, Mercer A, Kusecek B, Pohl A, Heuzenroeder M, Aaronson A, Sutton and A, Silver RP (1983) Six widespread bacterial clones among Escherichia coli Kl isolates. Infect Immun 39: 315–335

    PubMed  CAS  Google Scholar 

  2. Folch J, Lees M and Sloane-Stanley GH (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226: 497–505

    PubMed  CAS  Google Scholar 

  3. Gentry MK and Dalrymple JM (1980) Quantitative microtiter cytotoxicity assay for Shigella toxin. J Clin Micro 12: 361–366

    CAS  Google Scholar 

  4. Karkhanis YD, Zeltner JY, Jackson JJ and Carlo D (1978) A new and improved microassay to determine 2-keto-3-deoxyoctonate in lipopolysaccharides of gram-negative bacterial. Analytical Biochem. 85: 595–601

    Article  CAS  Google Scholar 

  5. Levner M, Wiener FP and Rubin BA (1977) Induction of Escherichia coli and Vibrio cholerae enterotoxins by an inhibitor of protein synthesis. Infect Immun 15: 132–137

    PubMed  CAS  Google Scholar 

  6. Lowry OW, Roberts NR, Leiner KY, Wu ML and Farr AL (1954) The quantitative histochemistry of brain I chemical methods. J Biol Chem 207: 1–17.

    PubMed  CAS  Google Scholar 

  7. Lugtenberg B, Meijers J, van der Hoek P, and van Alphen (1975) Electrophoretic resolution of the major outer membrane protein of Escherichia coli K12 into four bands. FEBS Lett 58: 254–258

    Article  PubMed  CAS  Google Scholar 

  8. Markwell MA K, Haar SM, Bieker LL, and Tolbert WE (1978) A modification of the Lowry procedure to simplify protein determinations in membrane and lipoprotein samples. Anal Biochem 87: 206–210

    Article  PubMed  CAS  Google Scholar 

  9. O’Brien AD, Chen ME, Wells JA, Farmer J, and Wachsmith TK (1985) Epidemiology of Shigella dysenteriae 1-like toxin production by Escherichia coli. In Advances Research on Cholera and Related Diarrheal Diseases. KTK Scientific Publisher, Tokyo, Japan (in press)

    Google Scholar 

  10. O’Brien AD, and LaVeck GD (1983) Purification and characterization of a Shigella dysenteriae 1 like toxin by Escherichia coli. Infect Immun 40: 675–683

    PubMed  Google Scholar 

  11. O’Brien AD, LaVeck GD, Thompson MR, and Formal SB (1982) Production of Shigella dysenteriae type 1 like cytotoxin by Escherichia coli. J Infect Dis 146: 675–683

    Google Scholar 

  12. O’Brien AD, Lively TA, Chen ME, Rothman SW, and Formal SB (1983) Escherichia coli 0157:H7 strains associated with haemorrhagic colitis in the United States produce a Shigella dysenteriae 1 (Shiga) like cytotoxin. Lancet i: 702

    Google Scholar 

  13. O’Brien AD, Lively TA, Chang TW, and Gorbach SL (1983) Purification of Shigella dysenteriae 1 (Shiga)-like cytotoxin from Escherichia coli 0157:H7 strain associated with hemorrhagic colitis. Lancet ii:573

    Google Scholar 

  14. O’Brien AD, Newland JW, Miller SF, Holmes RK, Smith HW, and Formal SB (1984) Shiga-like toxin converting phages from Escherichia coli strains that cause hemorrhagic colitis or infantile diarrhea. Science 226: 694–696

    Article  PubMed  Google Scholar 

  15. Rosenbusch JP, (1974) Characterization of the major envelope protein from Escherichia coli. J Biol Chem 249: 8019–8029

    PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Lehrstuhl für Med. Mikrobiologie und Immunologie, Ruhr-Universität Bochum, Germany

    H. Karch, P. Goroncy-Bermes & W. Opferkuch

  2. Pharma-Forschungszentrum, Bayer AG, Institut für Chemotherapie, Wuppertal, Germany

    H.-P. Kroll

  3. Uniformed Services University of the Health Sciences, Bethesda, USA

    A. O’Brien

  4. Institut für Medizinische Mikrobiologie und Immunologie, Universitäts-Krankenhaus Eppendorf, Germany

    H. Karch

Authors
  1. H. Karch
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  2. P. Goroncy-Bermes
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  3. W. Opferkuch
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  4. H.-P. Kroll
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  5. A. O’Brien
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Editor information

Editors and Affiliations

  1. Kinderklinik der Universität München, Lindwurmstraße 4, 8000, München 2, Germany

    Dieter Adam

  2. Institut für Medizinische Mikrobiologie, Freie Universität Berlin, Hindenburgdamm 27, 1000, Berlin 45, Germany

    Helmut Hahn

  3. Abteilung für Theoretische und Klinische Medizin, Ruhr-Universität Bochum, Universitätsstraße 150, 4630, Bochum 1, Germany

    Wolfgang Opferkuch

  4. Lehrstuhl für Medizinische Mikrobiologie und Immunologie, Ruhr-Universität Bochum, Universitätsstraße 150, 4630, Bochum 1, Germany

    Wolfgang Opferkuch

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© 1985 Springer-Verlag Berlin Heidelberg

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Karch, H., Goroncy-Bermes, P., Opferkuch, W., Kroll, HP., O’Brien, A. (1985). Subinhibitory concentrations of antibiotics modulate amount of Shiga-like toxin produced by Escherichia coli . In: Adam, D., Hahn, H., Opferkuch, W. (eds) The Influence of Antibiotics on the Host-Parasite Relationship II. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-70748-3_25

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  • DOI: https://doi.org/10.1007/978-3-642-70748-3_25

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-70750-6

  • Online ISBN: 978-3-642-70748-3

  • eBook Packages: Springer Book Archive

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