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The Influence of Antibiotics on the Host-Parasite Relationship II pp 16–27Cite as

Interaction of Aminoglycosides and Ciprofloxacin with Bacterial Membranes

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Abstract

Alterations in outer membrane permeability caused by polycationic antibiotics (e.g. polymyxins) are caused by displacement of divalent cations by competition; on the other hand EDTA removes divalent cations by chelation and thus causes outer membrane perturbation [1]. A wide range of outer membrane permeabilisation events is mediated by polymyxin B: the uptake of hydrophobic fluorescent compounds and nitrocefin respectively is enhanced and rod like projections (i.e. formation of outer membrane blebs) is caused by polymyxin B (2–10). Furthermore polymyxins cause a release of soluble constituents from washed cells of P.aeruginosa; there was an optimum concentration of polymyxin for maximum release of cell constituents, higher concentrations inhibiting the release [11,12]. Interaction of polymyxin B with the divalent cation-binding sites on LPS and permeabilisation of the outer membranes of gram-negative bacteria is reversible upon addition of magnesium or potassium.

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Author information

Authors and Affiliations

  1. Bayer Pharma Research Centre, Institute for Chemotherapy, P.O.Box 101 709, 5600, Wuppertal 1, Germany

    A. Dalhoff

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  1. A. Dalhoff
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Editor information

Editors and Affiliations

  1. Kinderklinik der Universität München, Lindwurmstraße 4, 8000, München 2, Germany

    Dieter Adam

  2. Institut für Medizinische Mikrobiologie, Freie Universität Berlin, Hindenburgdamm 27, 1000, Berlin 45, Germany

    Helmut Hahn

  3. Abteilung für Theoretische und Klinische Medizin, Ruhr-Universität Bochum, Universitätsstraße 150, 4630, Bochum 1, Germany

    Wolfgang Opferkuch

  4. Lehrstuhl für Medizinische Mikrobiologie und Immunologie, Ruhr-Universität Bochum, Universitätsstraße 150, 4630, Bochum 1, Germany

    Wolfgang Opferkuch

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© 1985 Springer-Verlag Berlin Heidelberg

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Dalhoff, A. (1985). Interaction of Aminoglycosides and Ciprofloxacin with Bacterial Membranes. In: Adam, D., Hahn, H., Opferkuch, W. (eds) The Influence of Antibiotics on the Host-Parasite Relationship II. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-70748-3_2

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  • DOI: https://doi.org/10.1007/978-3-642-70748-3_2

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-70750-6

  • Online ISBN: 978-3-642-70748-3

  • eBook Packages: Springer Book Archive

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