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DNA Hybridization Subtraction: Differences Detected Between BALB/cAnPt and BALB/cJax at the DNA Level

  • K. Huppi
  • A. von Deimling
  • J. Mushinski
  • M. Potter
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 122)

Abstract

A number of investigators have demonstrated DNA probes which map specifically to sex chromosomes X or Y (Kunkel et al. 1976; Schmeckpeper et al. 1979; Singh and Jones 1982; Lamar and Palmer 1984). The most recent report (Lamar and Palmer, 1984) has identified mouse chromosome Y specific sequences through the use of recombinant DNA techniques. Similar approaches have been made by D’Eustachio (1984) to obtain DNA fragments specific for mouse chromosome 12. We have adopted and modified a method of subtraction hybridization (Lamar and Palmer, 1984) to obtain recombinant DNA clones which distinguish closely related sublines of BALB/c inbred mice at autosomal loci.

Keywords

Autosomal Locus Major Histocompatibility Complex Locus Plasma Cell Neoplasm Specific Genotypic Difference Lymphocyte Alloantigen 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Anderson PN, Potter M (1969) Induction of plasma cell tumors in BALB/c mice with 2,6,10,14-tetramethy1 pentadecane (pristane). Nature 222: 994–995PubMedCrossRefGoogle Scholar
  2. D’Eustachio P (1984) A genetic map of mouse chromosome 12 composed of polymorphic DNA fragments. J Exp Med 160: 827–838PubMedCrossRefGoogle Scholar
  3. Forman J, Flaherty L (1978) Identification of a new CML target antigen controlled by a gene associated with the Qa-2 locus. Immunogenetics 6: 227–237CrossRefGoogle Scholar
  4. Huppi K, Jouvin-Marche E, Scott C, Potter M, Weigert M (1985) Genetic polymorphism at the k chain locus in mice: Comparisons of restriction enzyme hybridization fragments of variable and constant region genes. Immunogenetics (in press)Google Scholar
  5. Kunkel LM, Smith KD, Boyer SH (1976) Human Y-chromosome specific reiterated DNA. Science 191: 1189–1190PubMedCrossRefGoogle Scholar
  6. Lamar EE, Palmer E (1984) Y-encoded, species-specific DNA in mice: evidence that the Y chromosome exists in two polymorphic forms in inbred strains. Cell 37: 171–177PubMedCrossRefGoogle Scholar
  7. Merwin RM, Algire GH (1959) Induction of plasma cell neoplasms and fibrosarcomas in BALB/c mice carrying diffusion chambers. Proc Soc Exp Biol Med 101: 437–439PubMedGoogle Scholar
  8. Potter M, Boyce C (1962) Induction of plasma cell neoplasms in BALB/c mice with mineral oil adjuvants. Nature 193: 1086–1087PubMedCrossRefGoogle Scholar
  9. Schmeckpeper BJ, Smith KD, Dorman BP, Ruddle FH, Talbot Jr CC (1979) Partial purification and characterization of DNA from the human X chromosome. Proc Natl Acad Sci USA 76: 6525–6528PubMedCrossRefGoogle Scholar
  10. Singh L, Jones KW (1982) Sex reversal in the mouse (Mus musculus) is caused by a recurrent nonreciprocal crossover involving the X and an aberrant Y chromosome. Cell 28: 205–216PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1985

Authors and Affiliations

  • K. Huppi
  • A. von Deimling
  • J. Mushinski
  • M. Potter

There are no affiliations available

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