Abstract
Over the last few years, a great number of reports have been published dealing with in vitro chlorophyllous culture cells [3, 12, 13, 21, 30, 33, 37]. Green cell suspension cultures appear to be a suitable system for analyzing cytoplasm-plastid relationships during differentiation [1, 4, 18]. This experimental model is also useful for investigations of plant cell physiology in relation with different cell growth phases [21, 23]. Many studies have been reported using unicellular algae systems, but aspects of physiological events in actively dividing higher plant cells are not well documented [11, 26, 27]. The difficulties reside in the establishment and maintenance of stable green cells during the successive subcultures. Moreover, sucrose, the main carbon source present in cell culture media, is known to be implicated in the repression of chloroplast development and photosynthetic capacity [5, 14, 31]. Nowadays, selection procedure and improved culture conditions make it possible to obtain cell lines with functional chloroplasts [16, 35, 36] and high photosynthetic potentials [28, 32, 34, 40].
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Nato, A., Hoarau, J., Brangeon, J., Hirel, B., Suzuki, A. (1985). Regulation of Carbon and Nitrogen Assimilation Pathways in Tobacco Cell Suspension Cultures in Relation with Ultrastructural and Biochemical Development of the Photosynthetic Apparatus. In: Neumann, KH., Barz, W., Reinhard, E. (eds) Primary and Secondary Metabolism of Plant Cell Cultures. Proceedings in Life Sciences. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-70717-9_4
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DOI: https://doi.org/10.1007/978-3-642-70717-9_4
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