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Induction of Enzymes of Phytoalexin Synthesis in Soybean Cells by Fungal Elicitor

  • J. Ebel
  • M. R. Stäb
  • W. E. Schmidt
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)

Abstract

Higher plants have aquired effective defence mechanisms during evolution, which secure their survival in the presence of a large variety of infective microorganisms. Resistance mechanisms of plants are expressed at different levels in host-parasite interactions including preformed physical and chemical defence barriers as well as defences triggered by the invader [16]. One type of active response of plants to attempted infection is the production of low molecular weight antimicrobial compounds called phytoalexins. Considerable evidence supports the view that the phytoalexin response is one mechanism by which plants resist diseases [6, 12, 14, 21].

Keywords

Fungal Elicitor Soybean Cell Phytoalexin Synthesis Phytophthora Megasperma Culture Soybean Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Albersheim P, Valent BS (1978) Host-pathogen interactions in plants. Plants, when exposed to oligosaccharides of fungal origin, defend themselves by accumulating antibiotics. J Cell Biol 78:627–643PubMedCrossRefGoogle Scholar
  2. 2.
    Ayers AR, Ebel J, Finelli F, Berger N, Albersheim P (1976) Host-pathogen interactions. IX. Quantitative assays of elicitor activity and characterization of the elicitor present in the extracellular medium of cultures of Phytophthora megasperma var sofae. Plant Physiol 57:751–759PubMedCrossRefGoogle Scholar
  3. 3.
    Ayers AR, Ebel J, Valent B, Albersheim P (1976) Host-pathogen interactions. X. Fractionation and biological activity of an elicitor isolated from the mycelial walls of Phytophthora megasperma var sofae. Plant Physiol 57:760–765PubMedCrossRefGoogle Scholar
  4. 4.
    Ayers AR, Valent B, Ebel J, Albersheim P (1976) Host-pathogen interactions. XI. Composition and structure of wall-released elicitor fractions. Plant Physiol 57:766–774PubMedCrossRefGoogle Scholar
  5. 5.
    Burden RS, Bailey JA (1975) Structure of the phytoalexin from soybean. Phytochemistry 14: 1389–1390CrossRefGoogle Scholar
  6. 6.
    Darvill AG, Albersheim P (1984) Phytoalexins and their elicitors — A defense against microbial infection in plants. Annu Rev Plant Physiol 35:243–275CrossRefGoogle Scholar
  7. 7.
    Dewick PM (1982) Isoflavonoids. In: Harborne JB, Mabry TJ (eds) The flavonoids: Advances in research. Chapman and Hall, London, pp 535–640Google Scholar
  8. 8.
    Dieter P, Marmé D (1980) Ca2+ transport in mitochondrial and microsomal fractions from higher plants. Planta 150:1–8CrossRefGoogle Scholar
  9. 9.
    -Ebel J (1984) Induction of phytoalexin synthesis in plants following microbial infection or treatment with elicitors. In: Ory RL, Rittig FR (eds) Bioregulators, chemistry and uses, ACS Symp Ser, vol 257. Am Chem Soc, Washington DC, pp 257–271CrossRefGoogle Scholar
  10. 10.
    Ebel J, Ayers AR, Albersheim P (1976) Host-pathogen interactions. XII. Response of suspension-cultured soybean cells to the elicitor isolated from Phytophthora megasperma var sofae, a fungal pathogen of soybeans. Plant Physiol 57:775–779PubMedCrossRefGoogle Scholar
  11. 11.
    Ebel J, Schmidt WE, Loyal R (1984) Phytoalexin synthesis in soybean cells: Elicitor induction of phenylalanine ammonia-lyase and chalcone synthase mRNAs and correlation with phytoalexin accumulation. Arch Biochem Biophys 232:240–248PubMedCrossRefGoogle Scholar
  12. 12.
    Grisebach H, Ebel J (1978) Phytoalexins, chemical defense substances of higher plants? Angew Chem 17:635–657 (Int Ed Engl)Google Scholar
  13. 13.
    Hahn MG, Grisebach H (1983) Cyclic AMP is not involved as a second messenger in the response of soybean to infection by Phytophthora megasperma f sp glycinea. Z Naturforsch 38c: 578–582Google Scholar
  14. 14.
    Hahn MG, Bonhoff A, Grisebach H (1985) Quantitative localization of the phytoalexin glyce-ollin I in relation to fungal hyphae in soybean roots infected with Photophthora megasperma f sp glycinea. Plant Physiol 77:591–601PubMedCrossRefGoogle Scholar
  15. 15.
    Hille A, Purwin C, Ebel J (1982) Induction of enzymes of phytoalexin synthesis in cultured soybean cells by an elicitor from Phytophthora megasperma f sp glycinea. Plant Cell Rep 1: 123–127CrossRefGoogle Scholar
  16. 16.
    Horsfall JC, Cowling EB (eds) (1980) Plant disease, vol V. Academic Press, London New YorkGoogle Scholar
  17. 17.
    Kreuzaler F, Ragg H, Fautz E, Kuhn DN, Hahlbrock K (1983) UV-induction of chalcone synthase mRNA in cell suspension cultures of Petroselinum hortense. Proc Natl Acad Sci USA 80:2591–2593PubMedCrossRefGoogle Scholar
  18. 18.
    Leube J, Grisebach H (1983) Further studies on induction of enzymes of phytoalexin synthesis in soybean and cultured soybean cells. Z Naturforsch 38c:730–735Google Scholar
  19. 19.
    Lyne RL, Mulheim LJ (1978) Minor pterocarpinoids of soybean. Tetrahedron Lett 3127–3128Google Scholar
  20. 20.
    Lyne RL, Mulheim LJ, Leworthy DP (1976) New pterocarpinoid phytoalexins of soybean. J Chem Soc Chem Commun:497–498Google Scholar
  21. 21.
    Mansfield JW (1982) The role of phytoalexins in disease resistance. In: Bailey JA, Mansfield JW (eds) Phytoalexins. Blackie, Glasgow, pp 253–288Google Scholar
  22. 22.
    Moesta P, Grisebach H (1981) Investigation of the mechanism of phytoalexin accumulation in soybean induced by glucan or mercuric chloride. Arch Biochem Biophys 211:39–41PubMedCrossRefGoogle Scholar
  23. 23.
    Moesta P, Grisebach H (1981) Investigation of the mechanism of glyceollin accumulation in soybean infected by Phytophthora megasperma f sp glycinea. Arch Biochem Biophys 212: 462–467PubMedCrossRefGoogle Scholar
  24. 24.
    Schmelzer E, Börner H, Grisebach H, Ebel J, Hahlbrock K (1984) Phytoalexin synthesis in soybean (Glycine max). Similar time courses of mRNA induction in hypocotyls infected with a fungal pathogen and in cell cultures treated with fungal elicitor. FEBS Lett 172:59–63CrossRefGoogle Scholar
  25. 25.
    Sharp JK, McNeil M, Albersheim P (1984) The primary structures of one elicitor-active and seven elicitor-inactive hexa-β-D-glucopyranosyl-D-glucitols isolated from the mycelial walls of Phytophthora megasperma f sp glycinea. J Biol Chem 259:11321–11336PubMedGoogle Scholar
  26. 26.
    Zähringer U, Ebel J, Mulheim LJ, Lyne RL, Grisebach H (1979) Induction of phytoalexin synthesis in soybean. Dimethylallylpyrophosphate:trihydroxypterocarpan dimethylallyl transferase from elicitor-induced cotyledons. FEBS Lett 101:90–92PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1985

Authors and Affiliations

  • J. Ebel
  • M. R. Stäb
  • W. E. Schmidt
    • 1
  1. 1.Biologisches Institut II der UniversitätFreiburgGermany

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