Production of Alkaloids by Ergot (Claviceps fusiformis Lov.) on Pennisetum typhoides (Burm.) Stapf and Hubb. in Vitro
Ergot disease of pearl millet [Pennisetum typhoides (Burm.) Stapf and Hubb.] caused by Claviceps fusiformis Lov. results in the formation of sclerotia in place of normal grains. Most Claviceps sp. and strains, whether living as parasites or grown in axenic culture show growth phase, transition period, and finally alkaloid producing phase (idio-phase). Sclerotia are reported to contain clavine group of alkaloids. Like other indole alkaloids, ergot alkaloids are derived in part from tryptophan and methyl group of methionine. The ergot poisoning which may result from consumption of ergotted grains, is characterized by symptoms such as nausea, vomiting, diarrhoea, giddiness, and even death of human beings and livestock. Several investigations have been undertaken on the estimation and identification of alkaloids produced by Claviceps fusiformis in vivo and in axenic cultures [1–5].
KeywordsCallus Culture Pearl Millet Ergot Alkaloid Axenic Culture Indole Alkaloid
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- 1.Shinde PA, Bhide IP (1958) Ergot of bajra (Pennisetum typhoides) in Bombay state. Curr Sci 27: 499–500Google Scholar
- 3.Sivaprakasam K, Chinnadurai G, Krishnamurthy CS (1971) Alkaloid production by Claviceps microcephala on some varieties of pearl millet. Madras Agrie J 58: 431–432Google Scholar
- 5.Kumar A, Arya HC (1978) Estimation and identification of alkaloids produced by Claviceps fusiformis Loveless on some varieties of pearl millet. Curr Sci 47: 633–635Google Scholar
- 6.Ingram DS, Helgeson JP (eds) (1980) Tissue culture methods for plant pathologists. Blackwell, Oxford London, pp 272Google Scholar
- 8.Milholland RD (1962) Callus tissue from wheat for infection studies of Puccinia graminis var. tritici. Phytopathology (Abstr) 52: 21Google Scholar
- 12.Mukerji B, De NK (1944) A method for the assay of individual ergot Sclerotium. Curr Sci 13: 128Google Scholar
- 13.Vaidya HC, Desai JD (1981) Cell differentiation and alkaloid production in Claviceps sp. strain SD58. Indian J Exp Biol 19: 829–831Google Scholar
- 15.Bilgrami KS, Verma RN (1981) Physiology of fungi. Vikas, New Delhi, pp 507Google Scholar