Culturing Trophoblast Samples
Chorionic villi sampling (CVS) is now offered as an alternative to amniocentesis in many obstetric units in Europe and the United States for the prenatal diagnosis of fetal abnormality in pregnancies where the risk of genetic disease is high. A major advantage of this technique is that fresh villous material can be used for diagnosis (Williamson et al. 1981; Kazy et al. 1982; Old et al. 1982; Simoni et al. 1983), so that, if termination is indicated, it can be carried out in the first trimester at a time when abortion is safer and less distressing for the mother and more acceptable to the clinic staff involved in her antenatal care.
KeywordsAmniotic Membrane Chorionic Villus Maternal Cell Trimester Pregnancy Amniotic Fluid Cell
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- Alvarez H (1964) Morphology and physiopathology of the human placenta. Obstet Gynecol 28: 813–817Google Scholar
- Czepulkowski BH, Heaton DE, Coleman DV, Lilford RJ, Maxwell D, Horwell DH, Porter R (1985) Rapid and reliable culture of chorionic villous biopsies aspirated with ultrasound guidance. (in press)Google Scholar
- Department of Obstetrics and Gynecology (1975) Tietung Hospital of Anshan Iron and Steel Company, Anshan Fetal sex prediction by sex chromatin of chorionic villi cells during early pregnancy. Chin Med J (Engl) 1: 117–126Google Scholar
- Fensom AW, Jackson M, Sanguinetti N, Rodeck CH, Morsman JM, Coleman DV, Heaton D (1984) The use of chorionic villi for early prenatal diagnosis of metabolic disorders. J Med Genet 21: 142Google Scholar
- Goldberg MF, Chen AT, Young WA, Reidy J A (1980) First trimester fetal chromosomal diagnosis using endocervical lavage: a negative evaluation. Am J Obstet Gynaecol 138: 436–440Google Scholar
- Hahnemann N, Mohr J (1968) Genetic diagnosis in the embryo by means of biopsy from extraembryonic membranes. Bull Eur Soc Human Genet 2: 23–29Google Scholar
- Hahnemann N, Mohr J (1969) Antenatal foetal diagnosis in genetic disease. Bull Eur Soc Human Genet 3: 47–54Google Scholar
- Jones GES, Gey GO, Gey MK (1943) Hormone production by placental cells maintained in continuous culture. Bull Johns Hopkins Hosp 72: 26–38Google Scholar
- Niazi M (1982) PhD dissertation, University of LondonGoogle Scholar
- Rhine SA, Palmer CG, Thompson JF (1977) A simple alternative to amniocentesis for first trimester prenatal diagnosis. Liss, New York, pp 231–247 (Birth defects, original article series, vol X III no 3D )Google Scholar
- Thiede HA (1960) Studies of human trophoblast in tissue culture. Am J Obstet Gynaecol 79: 636–647Google Scholar