Brain Edema pp 258-262 | Cite as

Simultaneous Topographic Analysis of Brain Edema, Parenchymal Energy Metabolisms (NAD/NADH Redox State, Reduced Cytochrome aa3, ATP), and Intracellular Calcium During Cerebral Ischemia

  • N. Hayashi
  • T. Tsubokawa
  • S. Kimura
  • Y. Makiyama
  • M. Toeda
Conference paper


In cases of brain edema caused by cerebral ischemic insult it has been documented that cytotoxic edema occurs before vasogenic edema [3, 8] and that both types promote the development of secondary brain edema with concomitant metabolic changes and microcirculatory disturbances [5]. As a consequence, studies of brain edema have focused on the correlations between metabolic changes, intracellular ion changes, and vasogenic edema following cerebral ischemia. These parameters were simultaneously subjected to topographic analysis with reference to the same sliced brain by a laser photographic, computer-assisted method developed in our laboratory in collaboration with NIIC Laser Co. (4–16–13 Nishi Azabu Minato-ku, Tokyo, Japan). The block diagram is shown in Fig. 1.

Key words

Cerebral ischemia brain edema intracellular Ca mitochondrial energy metabolism laser photospectrometric analysis 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Blaustein MP, Ratzlaff RW, Schweitzer ES (1980) Control of intracellular calcium in presynaptic nerve terminals. Fed Proc 39:2790–2795PubMedGoogle Scholar
  2. 2.
    Crompton M, Moser R, Lüdi H, Carafoli E (1978) The interrelations between the transport of sodium and calcium in mitochondria of various mammalian tissues. Eur J Biochem 82:25–31PubMedCrossRefGoogle Scholar
  3. 3.
    Katzman R, Ciasen R, Klatzo I, Meyer JS, Pappius HM, Waltz AG (1977) Report of joint committee for stroke resources. 4: Brain edema in stroke. Stroke 8:512–540PubMedCrossRefGoogle Scholar
  4. 4.
    Kogure K, Alonso OF (1978) A pictorial representation of endogenous brain ATP by a bioluminescent method. Brain Res 154:273–284PubMedCrossRefGoogle Scholar
  5. 5.
    Little JR, Kerr FWL, Sundt TM Jr (1976) Microcirculatory obstruction in focal cerebral ischemia: an electron microscopic investigation in monkeys. Stroke 7:25–30CrossRefGoogle Scholar
  6. 6.
    Nathanson JA (1977) Cyclic nucleotides and nervous system function. Physiol Rev 57:157–256PubMedGoogle Scholar
  7. 7.
    Nicholls DG, Crompton M (1980) Mitochondrial calcium transport. FEBS Lett 111:261–268PubMedCrossRefGoogle Scholar
  8. 8.
    Schuier FJ, Hossmann KA (1980) Experimental brain infarcts in cats. II: Ischemic brain edema. Stroke 11:591–601CrossRefGoogle Scholar
  9. 9.
    Tsien RY, Rozzant T, Rink TJ (1982) Calcium homeostasis in intact lymphocytes: cytoplasmic free calcium monitored with a new intracellularly trapped fluorescent indicator. J Cell Biol 94:325–334PubMedCrossRefGoogle Scholar
  10. 10.
    Welsh FA (1980) In situ freezing of cat brain. In: Passonneau JV et al. (eds) Cerebral metabolism and neuronal function. Williams and Wilkins, BaltimoreGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1985

Authors and Affiliations

  • N. Hayashi
    • 1
  • T. Tsubokawa
  • S. Kimura
  • Y. Makiyama
  • M. Toeda
  1. 1.Department of NeurosurgeryNihon UniversityItabashi-ku, Tokyo 173Japan

Personalised recommendations