Advertisement

Modulation of a Potassium Channel in Aplysia Sensory Neurons: Role of Protein Phosphorylation

  • J. S. Camardo
  • M. J. Shuster
  • S. A. Siegelbaum
Conference paper
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)

Abstract

Serotonin (5-HT) produces a slow depolarizing postsynaptic potential in the mechanoreceptor sensory neurons of the abdominal ganglia of the marine snail Aplysia. This slow EPSP is accompanied by a decrease in resting membrane conductance, a broadening of the action potential duration, and an increase in transmitter release from the sensory neuron terminals. The increase in transmitter release is thought to underlie behavioral sensitization of the gill-withdrawal reflex (Kandel and Schwartz 1982). Voltage clamp experiments have shown that the primary effect of serotonin in the sensory neurons is to decrease a specific outward potassium membrane current (S current) that is distinct from the previously identified K+ currents in molluscan neurons (Klein et al. 1982).

Keywords

Sensory Neuron Single Channel Behavioral Sensitization Single Channel Conductance Single Channel Current 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Colquhoun D, Sakmann B (1983) Bursts of openings in transmitter activated ion channels. In: Sakmann B, Neher E (eds) Single channel recording. Plenum, New YorkGoogle Scholar
  2. Hamill OP, Marty A, Neher E, Sakmann B, Sigworth F (1981) Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch 391: 85–100PubMedCrossRefGoogle Scholar
  3. Hemmings HC, Greengard P, Lim Tung HY, Cohen P (1984) DARPP-32j, a dopamine-regulated neuronal phosphoprotein, is a potent inhibitor of protein phosphatase-1. Nature 310: 503–505PubMedCrossRefGoogle Scholar
  4. J.S. Camardo et al.: Modulation of a Potassium Channel in Aplysia Sensory Neurons Ingebritsen TS, Cohen P (1983) Protein phosphatases: properties and role in cellular regulation. Science 221: 331–337CrossRefGoogle Scholar
  5. Kandel ER, Schwartz JH (1982) Molecular biology of learning: Modulation of transmitter release. Science 218: 433–443Google Scholar
  6. Klein M, Camardo JS, Kandel ER (1982) Serotonin modulates a specific potassium current in the sensory neurons that show presynaptic facilitation in Aplysia. Proc Natl Acad Sci USA 79: 5713–5717PubMedCrossRefGoogle Scholar
  7. Krebs EG (1972) Protein kinases. Curr Top Cell Regul 5: 99–133PubMedGoogle Scholar
  8. Nestler EJ, Greengard P (1983) Protein phosphorylation in the brain. Nature 305:583–588Google Scholar
  9. Siegelbaum SA, Camardo JS, Kandel ER (1982) Serotonin and cAMP close single K channels in Aplysia sensory neurons. Nature 299: 413–417PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1986

Authors and Affiliations

  • J. S. Camardo
  • M. J. Shuster
  • S. A. Siegelbaum
    • 1
    • 2
  1. 1.Department of Pharmacology, Center for Neurobiology and Behavior, College of Physicians and SurgeonsColumbia UniversityNew YorkUSA
  2. 2.Department of Physiology, Center for Neurobiology and Behavior, College of Physicians and SurgeonsColumbia UniversityNew YorkUSA

Personalised recommendations