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The Muscarinic Receptor Subtype on Gastric Isolated Smooth Muscle Cells

  • S. M. Collins

Abstract

A recently proposed classification of muscarinic receptor subtypes in based on the regional differences in the affinities of these receptors for selective antagonists and agonists (Hammer and Giachietti 1983). Estimates of muscarinic receptor affinity are usually obtained from direct radioligand binding studies performed on subcellular fractions and from the measurement of biological responses of intact tissues. The extent to which these data are comparable is questionable since different experimental conditions pertain to the two approaches. This is particularly important with respect to the muscarinic receptor, the characteristics of which may be influenced by factors such as local concentrations of guanine nucleotides (Wei and Sulakahe 1979; Berrie et al. 1979; Rosenberger et al. 1979; Harden et al. 1982), muscarinic agonists (Klein et al. 1979), cations (Aronstam et al. 1978) and the ionic strength of the incubation medium (Burgen and Spero 1970). As a result, assessment of the affinity derived from radioligand binding studies performed on subcellular fractions in low ionic strength media may not be comparable to those derived from the measurement of biological responses of whole tissues suspended in physiological buffers.

Keywords

Muscarinic Receptor Circular Muscle Muscarinic Agonist Muscarinic Antagonist Muscarinic Receptor Subtype 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Aronstam RJ, Abood LG, Hoss W (1978) Mol Pharmacol 14: 575.PubMedGoogle Scholar
  2. Berrie CP, Birdsall NJM, Burgen ASV, Hulme EC (1979) Biochem Biophys Res Common 87: 1000.CrossRefGoogle Scholar
  3. Birdsall NJM, Hulme EC (1976) J Neurochem 27: 7.PubMedCrossRefGoogle Scholar
  4. Bitar KN, Zfass AM, Makhlouf GM (1979) Am J Physiol 37: E172.Google Scholar
  5. Burgen ASV, Spero L (1970) Br J Pharmacol 34:99.Google Scholar
  6. Collins SM, Gardner JD (1982) Am J Physiol 2243: G497.Google Scholar
  7. Goyal RK, Rattan S (1978) Prog Gastroenterol 74: 598.Google Scholar
  8. Hammer R, Giachietti A (1983) Life Sci 31: 2991.CrossRefGoogle Scholar
  9. Harden TK, Scheer AG, Smith MM (1982) Mol Pharmacol 21: 570.PubMedGoogle Scholar
  10. Klein WL, Nathanson N, Nirenberg M (1979) Biochem Biophys Res Common 90: 506.CrossRefGoogle Scholar
  11. Rosenberger LB, Roeske WR, Yamamura HI (1979) Eur J Pharmacol 56: 179.PubMedCrossRefGoogle Scholar
  12. Sakai Y, McLean J, Grover AK, Garfield RE, Fox JET, Daniel EE (1981) Comp J Physiol Pharmacol 59: 1260.CrossRefGoogle Scholar
  13. Wei J-W, Sulakhe PV (1980) Eur J Pharmacol 62: 345.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1985

Authors and Affiliations

  • S. M. Collins

There are no affiliations available

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