Isolation and Characterization of 5′-Nucleotidase from Avian Muscle Sources and its Interaction with Filamentous Actin
5′-Nucleotidase has been known for a long time to be an ectoenzyme concentrated in the plasma membrane of many eukaryotic cells. DePierre and Karnovsky (1974) first demonstrated its nature as an ectoenzyme and since then a vast amount of literature has been compiled concerning its enzymatic properties and presumed functional significance. In spite of efforts in many laboratories it has been impossible to assign a definitive function to this enzyme. It has been found, however, that adenosine, the product formed by its enzymatic action on 5′-AMP, can act as a local modulator of cellular functions in a number of different tissues, such as the vascular and central nervous systems (for a review see Arch and Newsholme 1978).
KeywordsSnake Venom Chicken Gizzard Lymphocyte Plasma Membrane AMPase Activity Bovine Pancreatic DNase
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