Abstract
The brains of 77 opossums (Didelphys virginiana) from Florida, California, Virginia, Texas, and North Carolina were used for the Golgi preparations in this study. Most pouch-young material was prepared for the rapid Golgi method (Morest and Morest 1966). The brain was fixed under hypothermia and sodium pentobarbital anesthesia either by immersion or intracardiac perfusion with osmium tetroxide and potassium dichromate. Many of these specimens were littermate series used in prior developmental studies (Morest 1968, 1969, 1970). However, the present report deals with morphological features representing relatively mature states of development, and they are comparable in both species unless otherwise specified. About half the brains were prepared under hypothermia and the same anesthetic but processed by the Golgi-Cox method (Van der Loos 1956; Ramon-Moliner 1970; Morest 1981). Both rapid Golgi and Golgi-Cox material was sectioned in each of the three cardinal anatomical or oblique planes.
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© 1986 Springer-Verlag Berlin Heidelberg
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Morest, D.K., Winer, J.A. (1986). Materials and Methods. In: The Comparative Anatomy of Neurons: Homologous Neurons in the Medial Geniculate Body of the Opossum and the Cat. Advances in Anatomy Embryology and Cell Biology, vol 97. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-70652-3_2
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DOI: https://doi.org/10.1007/978-3-642-70652-3_2
Publisher Name: Springer, Berlin, Heidelberg
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