Cell Volume Control and Ion Transport in a Mammalian Cell
There is general agreement that regulation of cellular volume reflects balance between passive and active ion movements across the cellular membrane with the colloid osmotic pressure of intracellular macrornolecules being offset by the extrusion of sodium ions from the cells. This pump-and-leak concept was developed about 25 years ago by Leaf (1959), Ussing (1960), and Tosteson and Hoffman (1960). In the course of time this concept still holds valid, but has proven to be somewhat simplistic. In recent years evidence has accumulated demonstrating that volume regulation in mammalian cells is achieved mainly via dynamic and controlled changes of the leak pathways (for references see Hoffmann 1978; Hoffmann et al. 1983; Hoffmann et al. 1984a) involving transient stimulation of normally dormant leak pathways (Hoffmann et al. 1984b). Furthermore, the “leaks” turns out to be a composite of a number of specific transport pathways involving also cotransport and exchange systems.
KeywordsPermeability Choline Taurine Furosemide Ouabain
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